商品编号


AG-40T-0284-C100



品牌


Adipogen



公司


Adipogen



公司分类


Proteins



Size

100 ?g

商品信息


More Information



Product Details



Synonyms

E3 Ubiquitin-protein Ligase Itchy Homolog; Itchy E3 Ubiquitin Protein Ligase; AIF4; AIP4; NAPP1; Atrophin-1-interacting Protein 4; NFE2-associated Polypeptide 1



Product Type

Protein



Properties



Source/Host

E. coli



Sequence

Human ITCH Isoform 2 (Accession Nr. Q96J02-2).



Crossreactivity

Human



Formulation

Liquid. In HEPES, NaCl, Glycerol and TCEP.



Other Product Data


Use:
HECT domain ubiquitin ligase (E3) that functions downstream of a ubiquitin-activating (E1) enzyme and a ubiquitin-conjugating (E2) enzyme to conjugate ubiquitin to substrate proteins. Demonstrates strong autoubiquitination activity
in vitro
. Reaction conditions will need to be optimized for each specific application. We recommend an initial protein concentration of 0.1-0.5?M.



Declaration

Manufactured by Boston Biochem



Shipping and Handling



Shipping

DRY ICE



Short Term Storage

-20°C



Long Term Storage

-80°C



Handling Advice

Aliquot to avoid freeze/thaw cycles.



Use/St
ABI
lity

Stable for at least 1 year after receipt when stored at -80°C.



Documents



MSD
S

No



Product Specification Sheet



Datasheet


Download PDF





The mammalian Itchy homolog, or ITCH, (also known as Atrophin-1-interacting protein 4 or AIP4) is a HECT domain class ubiquitin E3 ligase. ITCH/AIP4 ubiquitinates the phosphorylated form of dishevelled protein and promotes its degradation via the ubiquitin proteasome system, thereby inhibiting canonical Wnt signaling. The absence of ITCH/AIP4 has been shown to cause severe autoimmune disease in mice. Recent studies have identified multisystem autoimmune disease and morphologic and developmental abnormalities in human patients with ITCH/AIP4 deficiency, thus underscoring the importance of ITCH/AIP4 in many cellular processes. This ligase may catalyze K29-, K48-, and/or K63-linked polyubiquitin chain formation on a variety of reported targets.