商品编号


CHI-HR-200FGF2-C010



品牌


Adipogen



公司


Adipogen



公司分类


Proteins



Size

10 ?g

商品信息


More Information



Product Details



Synonyms

Basic Fibroblast Growth Factor; bFGF; Heparin Binding Growth Factor-2; HBGF-2



Product Type

Protein



Properties



Source/Host

E. coli



Sequence

Human FGF-2 (aa 143-288).



Crossreactivity

Human



BIOLOG
ical Activity

Measured by its
ABI
lity to stimulate the proliferation of balb/c3T3 cells. The ED50 for this effect is typically 0.1ng/ml, corresponding to a specific activity of 1x 10
7
units/mg.



MW

~17kDa (SDS-PAGE)



Purity

≥95% (SDS-PAGE)



Endotoxin Content

<0.1eu g="" purified="" protein="" (lal="" test;="" lonza).="">0.1eu>



Reconstitution

Reconstitute 10?g vial with 100 ?l sterile water to a concentration of 0.1mg/ml.
Reconstitute 50?g vial with 100 ?l sterile water to a concentration of 0.5mg/ml.
Add 1X PBS to the desired protein concentration.



Formulation

Lyophilized from 0.2?m-filtered solution in 50mM Tris-HCl, 400nM NaCl, pH8.0.



Other Product Data

NCBI reference NP_001997.5: FGF-2 (human)



Shipping and Handling



Shipping

BLUE ICE



Short Term Storage

+4°C



Long Term Storage

-20°C



Handling Advice

After reconstitution, prepare aliquots and store at -20°C.
Avoid freeze/thaw cycles.
PBS containing at least 0.1% BSA should be used for further dilutions.



Use/St
ABI
lity

Stable for at least 1 year after receipt when stored at -20°C.
Working aliquots are stable for up to 3 months when stored at -20°C.



Documents



MSD
S

No



Product Specification Sheet



Datasheet


Download PDF





FGF-2 (Basic fibroblast growth factor) belongs to the FGF superfamily that is made up of 22 different fibroblast growth factor genes. FGF-2 and FGF-1 (acidic FGF) are unique in that they do not follow the conventional signal sequence for secretion. FGF-2 is found in five isoforms via alternative initiation of translation. Signaling of FGF-2 occurs through the high-affinity tyrosine kinase receptors FGFR1-4. FGF-2 is a potent wide-spectrum mitogen whose overexpression is associated with immortalization and unregulated cell proliferation in many tumors. FGF-2 functions in angiogenesis, cell survival, tissue repair, embryonic development and inflammation. FGF-2 is widely used to maintain the self-renewal of human embryonic stem cells (ESCs) and to induce pluripotent stem cells. FGF-2 is an established neurogenic factor for proliferation and differentiation of multipotent neural stem cells both during development and in the adult mouse brain as well.