商品编号


M1010



品牌


UBPBio



公司


Ubiquitin-Proteasome Biotechnologies, LLC



公司分类


Ubiquitin / UBL Derivatives



Size

25 ?g




商品信息

A potent, irrevers
IBL
e and specific inhibitor of deubiquitinating enzymes (DUBs) that is prepared by chemical synthesis. It is N-terminally tagged with an HA-tag (YPYDVPDYA), which allows for sensitive identification or purification of DUBs since it is specifically recognized by anti-HA antibodies. The HA tag is separated from the Ub N-terminus by two aminohexanoic acid (Ahx) linkers for efficient recognition of the HA tag.



Additional Information






Product Name:

HA-Ub-VME



Also Known As:

N/A



Catalog No.:

M1010



Size:

25 ?g



Molecular Weight:

9913 Da by MS



Species:

Human



Source:

Synthetic



Stock:

Product in lyophilized powder form.



Concentration:

N/A



Quality Assurance:

≥95% by RP-HPLC



Storage:

Powder at ?20°C; solution at ?80°C. Avoid multiple freeze/thaw cycles.



PDF Data Sheet:

PDF Datasheet
，
MSD
S



NCBI RefSeq:

N/A



Image(s):

(Click image to enlarge)

















Coomassie-stained SDS-PAGE
Lane 1: HA-Ub-VME
Lane 2: Ubiquitin
Lane 3: Molecular weight
Marker
s






Shipping Method:

Room temperature shipping



References:

1. de Jong
et al
., (2012) ChemBioChem 13, 2251.
2. Borodovsky
et al
., (2001) EMBO J. 20, 5187. 3. Borodovsky
et al
., (2002) Chemistry and
BIOLOG
y 9, 1149.
4. El Oualid
et al
., (2010) Angew. Chem. Int. Ed. 49, 10149.




Details

A potent, irrevers
IBL
e and specific inhibitor of deubiquitinating enzymes (DUBs) that is prepared by chemical synthesis. It is N-terminally tagged with an HA-tag (YPYDVPDYA), which allows for sensitive identification or purification of DUBs since it is specifically recognized by anti-HA antibodies. The HA tag is separated from the Ub N-terminus by two aminohexanoic acid (Ahx) linkers for efficient recognition of the HA tag.

Sample Preparation:
Dissolve the powder in DMSO (e.g. add 1.25 μl DMSO to 25 μg powder to get 20 mg/ml or 2.25 mM DMSO stock) and add this DMSO stock to the required buffer (please note the order of addition). Typical reaction concentrations vary from 0.2 - 2 mM.
Images:
(Click image to enlarge)

















Coomassie-stained SDS-PAGE
Lane 1: HA-Ub-VME
Lane 2: Ubiquitin
Lane 3: Molecular weight
Marker
s