商品编号


421-10R-5



品牌


Lee Biosolutions



公司


Lee Biosolutions, Inc



公司分类


Proteins / Enzymes



Size

5 x 1 gram

商品信息



Description




Product Name

Recombinant Human Lysozyme



Source

Oryza sativa
(Rice)



Catalogue Number

421-10R



Purity

>
85% (SDS-PAGE)



Form

Lyophilized



Protein

>
0.75 mg protein/mg solid (Nitrogen Analyzer)



Specific Activity

>
100,000 U/mg protein



Moisture Content

10% (Karl Fischer)



Storage

2-8°C



Recertification

4 years



Solubility

Clear solution at 10 mg/mL PBS



Source Note

No animal components are used in the manufacturing process of this product, and it therefore considered an animal-free material.



Country of Origin

United States of America



CAS Number

12671-19-1



E.C. Number

3.2.1.17









Applications


Controls / Calibrators / Standards Immunoassays Cell Culture Assay Development / Validation Life Science




Overview


Lee Biosolutions
sells research and bulk quantities of Recombinant Human Lysozyme, expressed in rice and purified from an animal free system.
.
Lysozyme lyses the peptidoglycan polymer typically found in most bacterial cell walls. Recombinant Lysozyme has identical amino acid sequences as the native Lysozyme. In lysing bacterial cells, recombinant Lysozyme is added to the cell extraction buffer at a range of 0.05 to 0.5 mg/mL (7,000 to 70,000 U/mL).
Typically, the extraction buffer contains 100mM tris HCL, 2mM EDTA, 0.05% trion I-100, pH 8.0. The cell paste is s
USP
ended in extraction buffer and incubated for at least 15 minutes at room temperature. Alternatively, Triton X-100 at 1% and PMSF at 1mM can be added separately and the lysis solution is further incubated for at least 15 minutes at 37C. The lysed cell s
USP
ension is clarified by centrifugation at 20,000 g for at least 15 minutes. The extraction conditions and concentration of recombinant lyosozyme needed for optiomal cell lysis and protein recovery depends on various factors.
Not only does lysozyme have very important clinical implications, but it is also a useful tool in molecular
BIOLOG
y. The
ABI
lity of lysozyme to break down bacterial cell walls (cell lysis) is utilized by molecular
BIOLOG
ists. Cell lysis is necessary to extract recombinant proteins or DNA from bacterial cells. Human lysozyme is preferred to chicken lysozyme because it is up to four times more active. Also, the activity of chicken lysozyme has high lot-to-lot variation. Many recombinant protein production systems prefer an animal-free lysozyme to avoid the risk of infectious disease transmission.