商品编号


TG2023-2



品牌


TopoGEN



公司


TopoGEN



公司分类


Dna substrates



Size

20 ug

商品信息

?


pRYG DNA Product Description
This supercoiled plasmid is a pUC19 derivative in which the 206 bp SphI-NarI fragment of pUC19 was replaced with a 245 bp SphI-AccI fragment upstream of the human adult beta-globin gene promoter. The 245 bp fragment contains a 54 bp alternating repeat of purine/pyrimidine sequence that constitutes a strong eukaryotic topoisomerase II cleavage site.? Cleavages within the repeat are re
ADI
ly detected in the absence of topoisomerase II inhibitors such as m-AMSA or VM-26.? The sequence of the

plasmid is given in Table 2 of Spitzner et al (reference below).






REFERENCE
:
For detailed description of pRYG, its reactivity with Topo II and the sequence, see Spitzner et al., Nuc. Acid. Res. 18: 1-11 (1990)