商品编号


LS004910



品牌


Worthington



公司


Worthington Biochemical Corporation



公司分类


Products



Size

50 un

商品信息
Hydroxysteroid DehydrogenaseA purified powder obtained from adapted cells of a mutant strain. Activity on androsterone only, no activity exhibited on testosterone.Store at -20°C.REQUIRES SPECIAL SHIPPING: ICE PACK≥0.5 units per mg dry weight
Source:
Pseudomonas testosteroni
I.U.B.: 1.1.1.51 and 1.1.1.50
Hydroxysteroid dehydrogenases catalyze the interconversion of hydroxyl and carboxyl groups of steroids.
P.testosteroni
derived hydroxysteroid dehydrogenases are of two types: 3-α-hydroxysteroid dehydrogenase (α enzyme) and 3-β-hydroxysteroid dehydrogenase (β enzyme). The α enzyme has a molecular weight of 47,000 daltons. The α enzyme oxidizes only 3-α-hydroxysteroids of the C19, C21 and C24 series. It is inhibited by heavy metals and sulfhydryl-binding agents. The β enzyme catalyzes the oxidation of 3-β-hydroxysteroids of the C19 and C21 series, 17-β-hydroxysteroids of the C18, C19 and C21 series, as well as certain 16-β-hydroxysteroids. It is inhibited by heavy metals and reducing agents. The oxidation of testosterone is inhibited by 3,17-α-estr
ADI
ol and other 1,3,5-estr
ADI
ene derivatives.
Worthington
supplies two preparations: one from the regular
P. testosteroni
(
ATCC
11966) culture which produces both the α and the β enzymes, and a second from a mutant strain which produces almost exclusively the α enzyme. By using both, the β-hydroxysteroid can be determined by difference.
Technical Note
: STDHP and STDH contain both α and β activities. STDHMP, however, contains only α activity.
Unit Definition
: One Unit reduces one micromole of NAD per minute at 25°C, pH 9.0 using androsterone or testosterone as substrate.