商品编号


M0489S-500 reactions (50 μl vol)



品牌


纽英伦(NEB)



公司


New England Biolabs



公司分类






500 reactions (50 μl vol)


Concentration

2X


商品信息

Description:
One
Taq
? Hot Start Quick-Load? 2X Master Mix with GC Buffer is an optimized, ready-to-use blend of
Taq
and Deep Vent
R
? DNA Polymerases combined with an aptamer-based inhibitor. This enzyme blend is ideally suited to PCR applications from GC-rich templates, including pure DNA solutions, bacterial colonies, and
CDN
A products. The 3?→5? exonuclease activity of Deep Vent DNA Polymerase increases the fidelity and robust amplification of
Taq
DNA Polymerase (1). The hot start nature of the enzyme offers convenience with decreased interference from primer dimers and secondary products. The convenient master mix formulation contains dNTPs, MgSO
4
, buffer components and st
ABI
lizers as well as two commonly used tracking dyes for DNA gels. On a 1% agarose gel in 1X TBE, Xylene Cyanol FF migrates at ~4 kb and Tartrazine migrates at ~10 bp. Both dyes are present in concentrations that do not mask co-migrating DNA bands.
Amplification of a selection of sequences with varying GC content from human genomic DNA using OneTaq Hot Start DNA Polymerase. The presence or absense of an extended room temperature incubation does not affect performance. Ampicon size is indicated next to gel and GC content is indicated below gel.
Marker
M is the 1 kb DNA Ladder.
??
Highlights
Product Source
An
E. coli
strain that carries the
Taq
DNA Polymerase gene from
Thermus aquaticus
YT-1 and an
E. coli
strain that carries the Deep Vent
R
DNA Polymerase gene from
Pyrococcus
species GB-D.
Reagents Supplied
The following reagents are supplied with this product:
Store at (°C)
Concentration
One
Taq
?High GC Enhancer
10X
Notes:
Product specifications for individual components in the OneTaq Hot Start Quick-Load 2X Master Mix with GC Buffer are available separately.OneTaq Hot Start Quick-Load 2X Master Mix with GC Buffer is stable for fifteen freeze-thaw cycles when stored at -20°C.OneTaq Hot Start Quick-Load 2X Master Mix with GC Buffer is also stable for one month at 4°C, so for frequent use, an aliquot may be kept at 4°C.