商品编号


LS003319



品牌


Worthington



公司


Worthington Biochemical Corporation



公司分类


Products



Size

1 gm

商品信息
Pepsin ATwo times crystallized from dilute alcohol. A lyophilized powder.Store at 2-8°C.≥2,500 units per mg dry weight
Source:
Porcine Stomach
I.U.B.: 3.4.23.1
Pepsin is an acidic protease. Its inactive zymogen precursor, pepsinogen, is produced in the stomach mucosa. There are several pepsins designated A, B, C, and D. Pepsin A, the major component, has a molecular weight of 35,000 daltons and an optimum pH of approximately 1.0 for substrates such as casein or hemoglobin if the substrate is native protein. Pepsin cleaves proteins preferentially at carboxylic groups of aromatic amino acids such as phenylalanine and tyrosine. It will not cleave at bonds containing valine, alanine or glycine. Pepsin is assayed based on the method of Anson
J. Gen. Physiol., 22
, 79 (1938) using hemoglobin as the substrate. Pepsin is unstable above pH 6.
St
ABI
lity/Storage:
Pepsin for 1-2 years at 2-8°C.
Unit Definition
: One Unit releases 0.001 A280 as TCA soluble hydrolysis products from denatured hemoglobin per minute at 37°C. One FIP Unit, expressed as micromoles of tyrosine equivalents liberated per minute at 25°C, can be calculated as follows: 1
Worthington
Unit x 0.0071 = FIP units.
Related Products:
Collagenase
(CLS1-4/CLSPA)
Deoxyribonuclease I (DP/D/DCLS/D2/DPFF/DPRF)
Hemoglobin (HB)
Hyaluronidase (HSE/HSEP)
Neutral Protease (Dispase, NPRO)
Proteinase K (PROK)