商品编号


R0710S-1,250 units



品牌


纽英伦(NEB)



公司


New England Biolabs



公司分类


Restriction Endonucleases



Concentration

5,000 units/ml


Size

1,250 units

商品信息

Description:
CviKI-1 is a restriction endonuclease with 4 expected recognition sites as well as up to eleven relaxed non-cognate sites (star sites).
Product Source
An
E. coli
strain carrying the cloned and modified CviKI restriction gene derived from CA-1A, a
Chlorella
virus.
Reagents Supplied
The following reagents are supplied with this product:
Store at (°C)
Concentration
CutSmart? Buffer
-20
10X
Notes:
Based on the st
ABI
lity of the enzyme in the reaction, incubations longerthan 1 hr will not result in improved digestion, unless additionalenzyme is added. Please refer to Restriction endonuclease survival in a reaction for more information regarding this topic.For enzymes that cannot be heat-inactivated, we recommend using a column for cleanup (such as the Monarch? PCR & DNA Cleanup Kit), or running the reaction on an agarose gel and then extracting the DNA (we recommend Monarch Gel Extraction Kit), or performing a phenol/chloroform extraction.Star activity may result from extended digestion, high enzyme concentration or a glycerol concentration of >5%