商品编号


LS02128



品牌


Worthington



公司


Worthington Biochemical Corporation



公司分类


Products



Size

5x50 ug

商品信息
Protease, S. aureus, Sequencing GradeChromatographically purified according to Drapeau, G, et.al, JBC, 247, 6720 (1972). Supplied as a lyophilized powder containing 10?g or 50?g protein.Store at 2-8°C.N/A
Source:
Staph aureus V8
I.U.B.: 3.4.21.19
Protease
S. aureus
V8 (Endoproteinase-Glu-C) specifically cleaves peptide bonds on the COOH-terminal side of either aspartic or glutamic acids. In the presence of ammonium, the enzyme specificity is limited to glutamic sites. It has a molecular weight of 27,000 daltons and optimum pH's of 4.0 and 7.8 with hemoglobin as the substrate. Protease
S. aureus
V8 is inhibited by diisopropylfluorophosphate and monovalent anions such as F
-
, Cl
-
, CH
3
COO
-
and NO
3
. Enzyme activity is determined by the casein digestion assay described by Drapeau (
Methods Enzymol., 45
, 469, 1976).
St
ABI
lity/Storage:
Autolysis occurs at temperatures greater than 40°C. The enzyme is fully active in
USP
0.2% SDS. Stable for 12 months at 2-8°C.
Unit Definition
: One Unit causes a change of 0.001 A
280
nm per minute at 37°C, pH 7.8 using casein as the substrate.
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