商品编号


R0611S-1,000 units



品牌


纽英伦(NEB)



公司


New England Biolabs



公司分类


Restriction Endonucleases



Concentration

10,000 units/ml


Size

1,000 units

商品信息

Description:
PspGI: crystals (Adrienne Foster and Alan Friedman, Purdue University; John Pelletier, Krystel Le Coz and Shuang-yong Xu,
New England Biolabs
)
Product Source
An?
E. coli?
strain that carries the cloned PspGI gene from?
Pyrococcus?
species strain GI-H (H.W. Jannasch).
Reagents Supplied
The following reagents are supplied with this product:
Store at (°C)
Concentration
CutSmart? Buffer
-20
10X
Notes:
PspGI is a highly
Thermo
stable isoschizomer of BstNI and EcoRII.Activity at 85°C is 2-3 fold greater than at 75°C. At 95°C, PspGI has a half-life of 2 hours.Overdigestion of > 20 units of PspGI per ?g DNA and incubation times > 2 hours are not recommended.This enzyme is blocked by dcm methylation. More information can be found at Dam-Dcm and CpG Methylation.Single letter code for degenerate sites.Star activity may result from a glycerol concentration of >5%