Worthington/Reverse Transcriptase, Recombinant HIV/LS05003/200 un
酶
商品编号
LS05003
品牌
Worthington
公司
Worthington Biochemical Corporation
公司分类
Products
Size
200 un
商品信息
Reverse Transcriptase, Recombinant, HIVChromatographically purified dimeric form with M.W. of 66 kDa and 51 kDa. A solution in 10mM potassium phosphate, pH 7.4, 1mM DTT and 20% glycerol.Store at -20°C.REQUIRES SPECIAL SHIPPING: DRY ICE≥5,000 units per mg protein
Source:
Recombinant protein produced in E.coli
I.U.B.: 2.7.7.49
Chromatographically purified heterodimer composed of 66kDa and 51kDa subunits. Supplied as a solution in 10mM potassium phosphate, pH 7.4, 1mM DTT and 20% glycerol. Primarily for AIDS research purposes; this enzyme has less fidelity than the AMV enzyme in other applications such as the preparation of
CDN
A from mRNA for cloning purposes.
Unit Definition
: One Unit incorporates 1 nmole of tritiated d-TMP into acid precipitable products using poly(A)/oligo(dT)12-18 as the template/primer in 20 minutes at 37°C, pH 8.3.
Note: The current assay uses a more efficient template/primer combination that results in an approximate two-fold increase in specific activity compared to our previous assay. As a result,
Worthington
's package sizes contain the same amount of protein with twice as much activity based upon the new assay.
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产品货号:594.4