商品编号


M0205S-1,000 units



品牌


纽英伦(NEB)



公司


New England Biolabs



公司分类






1,000 units


Concentration

10,000 units/ml

商品信息

Description:
E. coli
DNA Ligase catalyzes the formation of a phosphodiester bond between the 5?-phosphate and the 3?-hydroxyl of two adjacent DNA strands in duplex DNA with cohesive ends. It is not appreciably active on blunt-ended substrates.
E. col
i DNA Ligase uses NAD as a cofactor and can be heat-inactivated.
E. coli
DNA Ligase is active at a range of temperatures (4° C – 37° C).?
Highlights
Specific Activity: 6,000 units/mg
Supplied with 10X Reaction Buffer
Product Source
Purified from?
E. coli
?strain containing a cloned
E. coli
DNA Ligase gene.
Reagents Supplied
The following reagents are supplied with this product:
Store at (°C)
Concentration
E. coli
DNA Ligase Reaction Buffer
-20
10X
Notes:
Requires NAD+ (nicotinamide adenine dinucleotide) as a cofactor, in contrast to other ligases which use rATP.Ligation of blunt-ended fragments is extremely inefficient. For ligation of blunt-ended fragments use T4 DNA Ligase.Does not ligate RNA to DNA (4).This enzyme ligates only DNA fragments with cohesive termini.