商品编号


CL-0005



品牌


Signosis



公司


Signosis



公司分类


miRNA Research




1 Ea


商品信息

Description:
The miR-17-92 cluster encoding miR-17-3p, miR-17-5p, miR-18a, miR-19a, miR-19b, miR--20a and miR-92 is highly expressed in tumor cells and are known to act as oncogenes. Expression of these miRNAs promotes cell proliferation, suppresses apoptosis of cancer cells, and induces tumor angiogenesis. New work reveals essential functions for these miRNAs not only in tumor formation but also during normal development of the heart, lungs, and immune system. A number of PCR methods have been developed for analyzing the expression level of miRNA. Based on its proprietary technology,
Signosis
has developed a highly sensitive and discriminative real-time PCR assay method for measuring multiple miRNA expression simultaneously, including
miR-17-3P, miR-17-5p, miR-18a, miR-19a, miR-19b, miR-20a, miR-92
. It implements oligo-ligation and SYBR green based real-time PCR. The assay can be used for quantitative analysis of miRNA expression in either total RNA or cell lysate without
CDN
A conversion.
Principle:
In the assay, the target miRNA molecule is hybridized with two oligos to form a RNA/DNA duplex. When the sequences are perfectly matched, they are aligned with the miRNA and the joint can be ligated with DNA ligase. A single nucleotide difference among miRNAs will block either the hybridization or the ligation. After the pair of oligos is ligated, the ligated molecules are subjected to real-time PCR analysis.

Data:
Real time PCR analysis of miR-17-92 cluster miRNAs in HeLa and MCF7.