商品编号


CL-0004



品牌


Signosis



公司


Signosis



公司分类


miRNA Research




1 Ea


商品信息

Description:
miRNAs are known to involve in multiple
BIOLOG
ical functions and abnormal expression is associated to human diseases such as cancers. They represent a promising new class of cancer bio
Marker
s. A number of PCR methods have been developed for analyzing the expression level of miRNA. Based on its proprietary technology,
Signosis
has developed a highly sensitive and discriminative real-time PCR assay method for measuring miRNA expression. It implements oligo-ligation and SYBR green based real-time PCR. The assay can be used for quantitative analysis of miRNA expression in either total RNA or cell lysate without
CDN
A conversion.
Principle:
In the assay, the target miRNA molecule is hybridized with two oligos to form a RNA/DNA duplex. When the sequences are perfectly matched, they are aligned with the miRNA and the joint can be ligated with DNA ligase. A single nucleotide difference among miRNAs will block either the hybridization or the ligation. After the pair of oligos is ligated, the ligated molecules are subjected to real-time PCR analysis.

Data:
Comparison of miRNA detection in HeLa RNA and Cell lysate. HeLa total RNA was prepared with Trizol reagent. HeLa cell lysate from 200 cells was prepared with Cell Lysis Buffer. The samples were subjected to miRNA real time PCR assay on a 7700 system.