商品编号


CL-0006



品牌


Signosis



公司


Signosis



公司分类


miRNA Research




1 Ea


商品信息

Description:
Let7 family is known to regulate proliferation and differentiation processes in species ranging from C- elegans to human. The mature forms (designated let-7a through let-7i ) differ at one to four positions from the let-7a sequence. The expression level of the isoforms is variable, which may play a diverse role in organ development and disease etiology. To distinguish these isoforms will help to elicit the mechanism of Let7 function. Based on its proprietary technology,
Signosis
has developed a highly sensitive and discriminative real-time PCR assay method for measuring miRNA expression of let7 isoforms (
Let7a, Let7b, Let7c, Let7d, Let7e, Let7f, Let7g and Let7i
) simultaneously. The assay implements oligo-ligation and SYBR green based real-time PCR, which can be used for quantitative analysis of miRNA expression in either total RNA or cell lysate without
CDN
A conversion.
Principle:
In the assay, the target miRNA molecule is hybridized with two oligos to form a RNA/DNA duplex. When the sequences are perfectly matched, they are aligned with the miRNA and the joint can be ligated with DNA ligase. A single nucleotide difference among miRNAs will block either the hybridization or the ligation. After the pair of oligos is ligated, the ligated molecules are subjected to real-time PCR analysis.

Data:




Analysis of Let7 Isoform Family with Real-Time PCR. 1fmol of 8 synthetic DNA (tem) for Let7a, Let7b, Let7c,Let7d, Let7e, Let7 were used as a template and incubated with Let7 oligo mix respectively. After the ligation, the individual ligated molecule was subject to real-time PCR analysis with 8 Let7 specific primer respectively.


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