商品编号


NB-0001



品牌


Signosis



公司


Signosis



公司分类


miRNA Research




1 Ea

商品信息

Description:
RNA samples are separated through gel electrophoresis and transferred onto a membrane. Expression of a specific miRNA is detected with a biotin-labeled probe. This regular Northern blot assay is suitable to most moderate to high expressors of miRNA. High sensitive Northern blot (NB-1001 and HP-XXXX) is needed to analyze low expressors of miRNA. The biotin-labeled probes contain two moieties – complementary sequence of the miRNA and a tag sequence. The tag sequence is then detected by an amplifier enriched with biotin molecules.
Principle:
RNA samples are separated through gel electrophoresis and transferred onto a membrane. Expression of a specific miRNA is detected with a biotin-labeled probe. This regular Northern blot assay is suitable to most moderate to high expressors of miRNA. High sensitive Northern blot (NB-1001 and HP-XXXX) is needed to analyze low expressors of miRNA. The biotin-labeled probes contain two moieties – complementary sequence of the miRNA and a tag sequence. The tag sequence is then detected by an amplifier enriched with biotin molecules.

Data:

Figure 1. miRNA northern blot validation of array results in human brain and liver with miR-122a and miR-125b probes.