商品编号


GWB-001011



品牌


GenWay



公司


GenWay



公司分类


Molecular Biology Products



Size

10?g

商品信息

Description:
pOET1N_6xHis is a baculovirus transfer vector designed for high level expression of foreign genes under the powerful AcMNPV polyhedron (
polh
) promoter. The vector encodes an optional N-terminal 6×His-Tagfusion sequence that may be utilized if the insert allows read-through in the correct re
ADI
ng frame. This greatly eases the purification of the recombinant protein since the 6×His-containing fusion proteins bind with high affinity to Ni-NTA Agarose. If required, the 6×His-Tagcan be removed by incubating the fusion protein in the presence of the proteinase cleavage enzyme Thrombin. pOET1N_6xHis is smaller than other available transfer vectors (4598 bp) which greatly facilitate the cloning steps. It has a Col E1 origin of replication and an ampicillin resistance gene for selection in
E. coli
. The
polh
sequences have been replaced by a multiple cloning site (MCS) containing unique restriction sites for insertion of the foreign gene in the correct orientation, as shown on the circular map. The coding strand of the MCS as transcribed from the
polh
promoter is shown below the circular map. The
Pac
I site at the end of the MCS provides translational stop codons in all three re
ADI
ng frames for expression of truncated proteins. The AcMNPV sequences flanking the gene in the transfer vectors MCS allow recombination with the viral DNA to insert the expression cassette into the
polh
locus. pOET1N_6xHis is compat
IBL
e with any baculovirus system that utilizes homologous recombination in insect cells.?
Additional Information:




Name

pOET 1N_6xHis transfer plasmid



Concentration

Lot Specific



St
ABI
lity

6 months



Storage

-20



Intended Use

Research Use Only