商品编号


GWB-100602



品牌


GenWay



公司


GenWay



公司分类


Molecular Biology Products



Size

100 reactions

商品信息

Description:
Baculovirus quantification now made easier than ever!

No more
?plaque assays

No more
?
ELISA
or immuno assays

No more
?virus extraction spin columns

Oxford Expression
Technologies has teamed up with
Agilent
Technologies to introduce A convenient ‘all reagents included’ virus extraction & quantification kit.

?

KEY FEATURES

? Titre accuracy comparable to plaque assay method

? Less than one hour hands-on time

? Virus DNA extraction and titration in under three hours

? Compat
IBL
e with any AcMNPV-based baculovirus system containing gp64 viral gene

? Single step virus DNA extraction: just add lysis solution (no spin columns)

? QPCR Primers/ Probe provided as a single reagent mix: just add water

?
Agilent
high performance, ultra sensitive master mix reagent included

? Plaque assay titred virus internal standards included for standard curve generation

?

OVERVIEW OF PROTOCOL

See image for a schematic of the protocol

1. Virus DNA is extracted from recombinant? viruses of unknown titre and from a series of dilutions of the virus internal standard (no columns are needed, simply add lysis solution to the virus sample and place into a
Thermo
cycler)

2. QPCR is carried out to determine the C
t?
values for the virus DNA samples and internal standards (realtime PCR machine)

3. The titres of the virus DNA samples are determined by plotting their C
t
?values onto a standard curve generated using virus internal standards (provided in the kit)

?

KIT CONTENTS

1. Lysis buffer

2. Primer/ Probe mix

3. QPCR master mix

4. Virus internal standard

5. DNA positive control

6. RNase free water

7. User Guide

The kit contains reagents adequate for 100 reactions. This allows for upto 24 titrations in triplicate and 3 standard curves.?

Please note that the kit is shipped on dry ice.?

Additional Information:




Name

baculoQUANT All-in-one virus extraction & titration kit



Concentration

Lot Specific



Storage

-20C



Intended Use

Research Use Only