商品编号


GWB-001012



品牌


GenWay



公司


GenWay



公司分类


Molecular Biology Products



Size

10?g

商品信息

Description:
pOET1C_6xHis is a baculovirus transfer vector designed for high level expression of foreign genes under the powerful AcMNPV polyhedron (
polh
) promoter. The vector encodes an optional C-terminal 6×His-Tagfusion sequence that may be utilized. This greatly eases the purification of the recombinant protein since the 6×His-containing fusion proteins bind with high affinity to Ni-NTA Agarose. pOET1C_6xHis has a Col E1 origin of replication and an ampicillin resistance gene for selection in
E. coli
. The
polh
sequences have been replaced by a multiple cloning site (MCS) containing unique restriction sites for insertion of the foreign gene in the correct orientation, as shown on the circular map. The coding strand of the MCS as transcribed from the
polh
promoter is shown below the circular map. The AcMNPV sequences flanking the gene in the transfer vectors MCS allow recombination with the viral DNA to insert the expression cassette into the
polh
locus. pOET1C_6xHis is compat
IBL
e with any baculovirus system that utilizes homologous recombination in insect cells.?
Additional Information:




Name

pOET 1C 6xHis transfer plasmid



Concentration

Lot Specific



St
ABI
lity

1 year



Storage

-20C



Intended Use

Research Use Only