商品编号


ax0321



品牌


Axolbio



公司


Axolbio



公司分类


Human Cells




1 Ea

商品信息

Description:
Human iPSC-Derived Neural Stem Cells that have been genetically edited using CRISPR-Cas9 technology to introduce the R406W mutation (CGG>TGG) into the
MAPT
gene. This line is heterozygous for the R406W mutation where one allele contains the mutation and the other allele is wild type at the locus.

The R406W mutation in MAPT has been implicated in familial frontotemporal dementia and parkinsonism (Hutton
et al.
, 1998; Behnam
et al.
, 2015) and has been seen in patients with clinical presentation resembling Alzheimer's disease (Rademakers
et al.
, 2003; Lindquist
et al.
, 2008). The R406W mutation reduces the
ABI
lity of MAPT/tau to bind to microtubules
in vitro
(Hong
et al.
, 1998).

Click here to see more data on Axol Neural Stem Cells
Product Specification:


Starting material

Dermal fibroblast



Donor gender

Female



Donor age at sampling

64 yrs



Karyotype

Normal



Reprogramming method

Episomal vector



Induction method

Monolayer & chemically defined medium



Genetic modification

Heterozygous for the MAPT R406W mutation (CGG>TGG)



Genetic modification

Contains a puromycin resistance cassette (intronic)



Size

≥1.5 million cells



Kit components

1 vial of Neural Stem Cells (≥1.5 million cells) and 1 bottle of Neural Plating-XF Medium (30 mL)



Growth properties

Adherent



Shipping conditions

Dry ice



Storage conditions

Liquid nitrogen