Innovative Research/Human PAI-1 N-terminal cysteine mutant, latent fraction/INTCYSPAI-L/0.5mg
天然蛋白
商品编号
INTCYSPAI-L
品牌
Innovative Research
公司
Innovative Research
公司分类
Proteins
Size
0.5mg
商品信息
Product Description
Human PAI-1 N-terminal cysteine mutant, latent fraction
Wild type PAI-1 cloned with a cysteine residue at the N-terminus (patent pending). The latent fraction is < 0.4 percent active as determined by titration with HMW tc-urokinase and SDS PAGE. The protein is supplied in buffer with 1 mM DTT to prevent dimerization. To label with thiol reactive dye, buffer exchange into degassed 0.05M Sodium Phosphate; 0.1M NaCl; 1mM EDTA; pH 6.6 by desalting column. Collect into 10 fold molar excess of maleimide or iodoacetamide dye and incubate for 1 hour at room temperature. Remove free dye by desalting, ammonium sulfate precipitation, or dialysis.
References:
产品货号:9824.6