Innovative Research/Human PAI-1 N-terminal cysteine mutant, latent fraction/INTCYSPAI-L/1.0mg-氯霉素检测

Innovative Research/Human PAI-1 N-terminal cysteine mutant, latent fraction/INTCYSPAI-L/1.0mg

$Innovative Research/Human PAI-1 N-terminal cysteine mutant, latent fraction/INTCYSPAI-L/1.0mg$

商品编号 INTCYSPAI-L 品牌 Innovative Research 公司 Innovative Research 公司分类 Proteins Size 1.0mg 商品信息 Product Description Human PAI-1 N-terminal cysteine mutant, latent fraction Wild type PAI-1 cloned with a cysteine residue at the N-terminus (patent pending). The latent fraction is < 0.4 percent active as determined by titration with HMW tc-urokinase and SDS PAGE. The protein is supplied in buffer with 1 mM DTT to prevent dimerization. To label with thiol reactive dye, buffer exchange into degassed 0.05M Sodium Phosphate; 0.1M NaCl; 1mM EDTA; pH 6.6 by desalting column. Collect into 10 fold molar excess of maleimide or iodoacetamide dye and incubate for 1 hour at room temperature. Remove free dye by desalting, ammonium sulfate precipitation, or dialysis. References:

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产品货号：16179.2