商品编号


AG-20B-0010-C100



品牌


Adipogen



公司


Adipogen



公司分类


Antibodies



Size

100 ?g


商品信息


More Information



Product Details



Synonyms

Z-DNA-binding Protein 1; Tumor Stroma and Activated Macrophage Protein DLM-1; DNA-dependent Activator of IFN-regulatory Factors; DAI



Product Type

Monoclonal Antibody



Properties



Clone

Zippy-1



Isotype

Mouse IgG2a



Source/Host

Purified from concentrated hybridoma tissue culture supernatant.



Immunogen/Antigen

Recombinant mouse ZBP1 (aa 1-411).



Application


Immunocytochemistry:
(1:500) (see reference 1)

Immunoprecipitation:
(1:200)

Western Blot:
(1:1’000)



Crossreactivity

Human
Mouse



Specificity

Recognizes human and mouse ZBP1.



Purity

≥95% (SDS-PAGE)



Purity Detail

Protein G-affinity purified.



Concentration

1mg/ml



Formulation

Liquid. In PBS containing 10% glycerol and 0.02% sodium azide.



Shipping and Handling



Shipping

BLUE ICE



Short Term Storage

+4°C



Long Term Storage

-20°C



Handling Advice

After opening, prepare aliquots and store at -20°C.
Avoid freeze/thaw cycles.



Use/St
ABI
lity

Stable for at least 1 year after receipt when stored at -20°C.



Documents



MSD
S


Download PDF



Product Specification Sheet



Datasheet


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ZBP1 is an interferon induc
IBL
e protein that binds to cytoplasmic DNA and plays a role in host defense against tumors and pathogens. ZBP1 is highly expressed in lymphatic tissues including lymph node, leukocytes, tonsil, bone marrow and spleen. ZBP1 is an innate immune sensor of Influenza A virus (IAV). ZBP1 regulates NLRP3 (nucleotide and oligomerization domain, leucine-rich repeat-containing protein family, pyrin domain containing 3) inflammasome activation as well as induction of different types of cell death (apoptosis, necroptosis and pyroptosis) during IAV infection.


Product References

ZBP1/DAI is an innate sensor of influenza virus triggering the NLRP3 inflammasome and programmed cell death pathways: T. Kuriakose, et al.; Sci. Immunol.
1,
aag2045 (2016)

DAI senses Influenza A virus genomic RNA and activates RIPK3-dependent cell death: R.J. Thapa, et al.; Cell Host & Microbe
20
, 674 (2016)

RIPK1 counteracts ZBP1-mediated necroptosis to inhibit inflammation: J. Lin, et al.; Nature
540,
124 (2016)

RIPK1 inhibits ZBP1-driven necroptosis during development: K. Newton, et al.; Nature
540,
129 (2016)

IRF1 Is a Transcriptional Regulator of ZBP1 Promoting NLRP3 Inflammasome Activation and Cell Death during Influenza Virus Infection: T. Kuriakose, et al.; J. Immunol. ahead of print (2018)