商品编号


AG-27B-6004PF-C100



品牌


Adipogen



公司


Adipogen



公司分类


Antibodies



Size

100 ?g


商品信息


More Information



Product Details



Synonyms

ZIKV E Protein



Product Type

Recombinant Antibody



Properties



Clone

ZKA64



Isotype

Human IgG1κ



Immunogen/Antigen

Zika Virus



Label/Conjugates

Preservative Free



Application


Functional Application (Neutralizing):
Neutralizes Zika Virus (ZIKV) with an IC
50
of ~93ng/ml. Also enhances ZIKV infection in non-permissive K562 cells at a broad range of concentrations (not above 1?g/ml).
Optimal conditions should be determined individually.



Crossreactivity

Virus



Specificity

Recognizes and binds specifically to the EDIII domain of the Zika Virus envelope protein (ZIKV E). It does not cross-react with Dengue virus envelope protein (DENV E).



Purity

≥95% (SDS-PAGE)



Purity Detail

Protein A-affinity purified.



Endotoxin Content

<0.001eu mu;g="">0.001eu>



Concentration

1mg/ml



Formulation

Liquid. In PBS.



Other Product Data


Source:
EBV-immortalized memory B cells derived from ZIKV-infected, DENV-na?ve human donors.



Shipping and Handling



Shipping

BLUE ICE



Short Term Storage

+4°C



Long Term Storage

-20°C



Handling Advice

Avoid freeze/thaw cycles.



Use/St
ABI
lity

Stable for at least 1 year after receipt when stored at -20°C.
Stable for at least 3 months after receipt when stored at +4°C.



Documents



MSD
S


Download PDF



Product Specification Sheet



Datasheet


Download PDF





The main route of Zika Virus (ZIKV) infection is through bites by Aedes mosquitos, but the virus may also be sexually and vertically transmitted. Although most of the ZIKV infections are asymptomatic or cause only mild symptoms, there is evidence that ZIKV infection can lead to neurological complications, such as Guillain-Barré syndrome in adults and congenital birth defects, including microcephaly in the developing fetus, likely through its
ABI
lity to infect human neural
Progen
itor cells. Whereas flavivirus envelope (E) proteins mediate fusion and are the main target of neutralizing antibodies, the non-structural protein 1 (NS1) is secreted by infected cells and is involved in immune evasion and pathogenesis. The E protein contains three domains: EDI involved in the conformational changes required for viral entry; EDII containing the fusion loop; and EDIII which may be involved in binding to cellular receptors. The best specific neutralizing antibodies to ZIKV are directed against the domain EDIII of ZIKV.


Product References

Specificity, cross-reactivity, and function of antibodies elicited by Zika virus infection: K. Stettler, et al.; Science
353
, 823 (2016)