商品编号


AG-40B-0126-3010



品牌


Adipogen



公司


Adipogen



公司分类


Proteins



Size

3 x 10 ?g


商品信息


More Information



Product Details



Synonyms

FliC (NLRC4 Mutant)



Product Type

Protein



Properties



Source/Host

E. coli



Sequence

The native flagellin [FliC] from Salmonella enterica
SERO
var Typhimurium strain
ATCC
14028 (aa 1-495) contains a small deletion at the C-terminus of the protein required for NLRC4 activation and is fused at the N-terminus to a His-tag.



Crossreactivity

Human
Mouse



Specificity

Detected by human and mouse TLR5 (Toll-like receptor 5). Not detected by mouse NLRC4 (Nod-like receptor [NLR] family caspase activation and recruitment domain [CARD] domain-containing protein 4).



BIOLOG
ical Activity

Activation of TLR5 in human epithelial cell assays based on NF-κB luciferase fusions,
in vivo
on expression of CCL20 or IL-6. TLR5 can be stimulated
in vitro
by Flagellin (NLRC4 mutant) (rec.) at a concentration range of 5-50ng/ml and
in vivo
at a starting concentration of 0.1-1?g/mouse. Does not activate NLRC4 as tested in bone-marrow mouse macrophages (in the presence of streptolysin O (SLO)). It can be used as negative control for NLRC4 signaling.



Purity

≥95% (SDS-PAGE)



Endotoxin Content

<0.01eu mu;g="" purified="" protein="" (lal="" test;="" lonza).="">0.01eu>



Concentration

0.1mg/ml after reconstitution.



Reconstitution

Reconstitute with 100μl sterile water.



Formulation

Lyophilized. Contains PBS.



Other Product Data

NCBI link NP_460912.1: Flagellin [Salmonella enterica subsp. enterica
SERO
var Typhimurium str. LT2]



Shipping and Handling



Shipping

BLUE ICE



Short Term Storage

+4°C



Long Term Storage

-20°C



Handling Advice

After reconstitution, prepare aliquots and store at -20°C.
Avoid freeze/thaw cycles.



Use/St
ABI
lity

Stable for at least 6 months after receipt when stored at -20°C.



Documents



MSD
S


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Product Specification Sheet



Datasheet


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Flagellin is the subunit protein which polymerizes to form the filaments of bacterial flagella. It activates the innate immune system through the receptor Toll-like Receptor 5 (TLR5) or the intracellular NLRC4 protein. The Flagellin (NLRC4 Mutant)(rec.) is only detected by TLR5 not by NLRC4.