商品编号


AG-40B-0125-3010



品牌


Adipogen



公司


Adipogen



公司分类


Proteins



Size

3 x 10 ?g


商品信息


More Information



Product Details



Synonyms

FliC



Product Type

Protein



Properties



Source/Host

E. coli



Sequence

The native flagellin [FliC] from Salmonella enterica
SERO
var Typhimurium strain
ATCC
14028 (aa 1-495) is fused at the N-terminus to a His-tag.



Crossreactivity

Human
Mouse



Specificity

Detected by human and mouse TLR5 (Toll-like receptor 5) and mouse NLRC4 (Nod-like receptor [NLR] family caspase activation and recruitment domain [CARD] domain-containing protein 4).



BIOLOG
ical Activity

Activation of TLR5 in human epithelial cell assays based on NF-κB luciferase fusions,
in vivo
on expression of CCL20 or IL-6. TLR5 can be stimulated
in vitro
by Flagellin (rec.) at concentration range of 5-50ng/ml and
in vivo
at a starting concentration of 0.1-1?g/mouse. Inflammasome NLRC4 can be activated at a concentration range of 1-5?g/ml on bone-marrow macrophages in the presence of streptolysin O (SLO, 25?g/ml), a protein that allows delivery of exogenous molecules into the cytosol of living cells.



Purity

≥95% (SDS-PAGE)



Endotoxin Content

<0.01eu mu;g="" purified="" protein="" (lal="" test;="" lonza).="">0.01eu>



Concentration

0.1mg/ml after reconstitution.



Reconstitution

Reconstitute with 100μl sterile water.



Formulation

Lyophilized. Contains PBS.



Other Product Data

UniProt link P06179: Flagellin (Salmonella typhimurium)



Shipping and Handling



Shipping

BLUE ICE



Short Term Storage

+4°C



Long Term Storage

-20°C



Handling Advice

After reconstitution, prepare aliquots and store at -20°C.
Avoid freeze/thaw cycles.



Use/St
ABI
lity

Stable for at least 6 months after receipt when stored at -20°C.



Documents



MSD
S


Download PDF



Product Specification Sheet



Datasheet


Download PDF





Flagellin is the subunit protein which polymerizes to form the filaments of bacterial flagella. It activates the innate immune system through the receptor Toll-like Receptor 5 (TLR5) and the intracellular protein NLRC4 (NLR family CARD domain-containing protein 4).