商品编号


AG-40T-0262MC-C025



品牌


Adipogen



公司


Adipogen



公司分类


Proteins



Size

25 ?g

商品信息


More Information



Product Details



Synonyms

γ-aminobutyric Acid Receptor-associated Protein-like 2; GABA(A) Receptor-associated Protein-like 2; Ganglioside Expression Factor 2; GEF-2; General Protein Transport Factor p16; Golgi-associated ATPase Enhancer of 16 kDa; MAP1 Light Chain 3-related



Product Type

Protein



Properties



Source/Host

E. coli



Sequence

Human GATE-16 (Accession Nr. NP_009216) fused to a N-terminal HA-tag and conjugated to AMC.



Crossreactivity

Human



Label/Conjugates

AMC



Formulation

Liquid. In MOPS and NaCl.



Other Product Data


Use:
Fluorogenic substrate for some ATG-specific isopeptidases, based on the C-terminal derivatization of ATG8 with 7-amido-4-methylcoumarin (AMC). Useful for studying such enzyme activities when detection sensitivity or continuous monitoring of activity is essential. Release of AMC fluorescence can be monitored with an excitation wavelength of 380nM and an emission wavelength of 460nM. Reaction conditions will need to be optimized for each specific application. We recommend an initial protein concentration of 0.1-1?M.



Declaration

Manufactured by Boston Biochem



Shipping and Handling



Shipping

DRY ICE



Short Term Storage

-20°C



Long Term Storage

-80°C



Handling Advice

Aliquot to avoid freeze/thaw cycles.
Protect from light.



Use/St
ABI
lity

Stable for at least 1 year after receipt when stored at -80°C.



Documents



MSD
S

No



Product Specification Sheet



Datasheet


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Golgi-associated ATPase Enhancer of 16 kDa (GATE-16), also known as Apg8p2 and GABARAPL2, is a 117 amino acid (aa) polypeptide and a member of the autophagy-related 8 (Atg8) family of proteins. GATE-16 has 100% aa sequence identity with its mouse and rat orthologs, and is orthologous to the yeast Atg8. Atg8 family members show structural similarity with ubiquitin, but lack aa sequence similarity. GATE-16 is best known for its role in autophagy. GATE-16 covalently attaches to phosphatidylethanolamine (PE) the phagophore (autophagosome precursor) membrane using a ubiquitin-like conjugation system that includes ubiquitin-activating (E1)-, ubiquitin-conjugating (E2)-, and ubiquitin ligase (E3)-like enzymes. Here it is involved in the later stages of autophagosome formation. It may also be involved in cargo recruitment to autophagosomes.