商品编号


AG-40T-0263-C500



品牌


Adipogen



公司


Adipogen



公司分类


Proteins



Size

500 ?g

商品信息


More Information



Product Details



Synonyms

γ-aminobutyric Acid Receptor-associated Protein-like 2; GABA(A) Receptor-associated Protein-like 2; Ganglioside Expression Factor 2; GEF-2; General Protein Transport Factor p16; Golgi-associated ATPase Enhancer of 16 kDa; MAP1 Light Chain 3-related



Product Type

Protein



Properties



Source/Host

E. coli



Sequence

Human GATE-16 (Accession Nr. NP_009216) fused to a N-terminal His-tag.



Crossreactivity

Human



Formulation

Liquid. In 50mM HEPES pH 8.0, 100mM NaCl, 10% glycerol, 1mM DTT.



Other Product Data


Use:
Can be conjugated to substrate proteins via the subsequent actions of an Apg-activating (E1) enzyme, an Apg-conjugating (E2) enzyme, and an Apg ligase (E3). Reaction conditions will need to be optimized for each specific application. We recommend an initial protein concentration of 10-50?M.



Declaration

Manufactured by Boston Biochem



Shipping and Handling



Shipping

DRY ICE



Short Term Storage

-20°C



Long Term Storage

-80°C



Handling Advice

Aliquot to avoid freeze/thaw cycles.



Use/St
ABI
lity

Stable for at least 1 year after receipt when stored at -80°C.



Documents



MSD
S

No



Product Specification Sheet



Datasheet


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Golgi-associated ATPase Enhancer of 16 kDa (GATE-16), also known as Apg8p2 and GABARAPL2, is a 117 amino acid (aa) polypeptide and a member of the autophagy-related 8 (Atg8) family of proteins. GATE-16 has 100% aa sequence identity with its mouse and rat orthologs, and is orthologous to the yeast Atg8. Atg8 family members show structural similarity with ubiquitin, but lack aa sequence similarity. GATE-16 is best known for its role in autophagy. GATE-16 covalently attaches to phosphatidylethanolamine (PE) the phagophore (autophagosome precursor) membrane using a ubiquitin-like conjugation system that includes ubiquitin-activating (E1)-, ubiquitin-conjugating (E2)-, and ubiquitin ligase (E3)-like enzymes. Here it is involved in the later stages of autophagosome formation. It may also be involved in cargo recruitment to autophagosomes.