商品编号


AG-40T-0264-C025



品牌


Adipogen



公司


Adipogen



公司分类


Proteins



Size

25 ?g

商品信息


More Information



Product Details



Synonyms

γ-aminobutyric Acid Receptor-associated Protein-like 2; GABA(A) Receptor-associated Protein-like 2; Ganglioside Expression Factor 2; GEF-2; General Protein Transport Factor p16; Golgi-associated ATPase Enhancer of 16 kDa; MAP1 Light Chain 3-related



Product Type

Protein



Properties



Source/Host

E. coli



Sequence

Human GATE-16 (Accession Nr. NP_009216) fused to a N-terminal HA-tag and tagged to vinyl sulfone.



Crossreactivity

Human



Formulation

Liquid. In 50mM Hepes pH 7.5, 100mM NaCl, 10% glycerol.



Other Product Data


Use:
Potent, irrevers
IBL
e and specific inhibitor of Agp8-specific isopeptidases (such as Apg4B). Apg4B activities include the processing of Apg8 precursor proteins and the removal of Apg8 proteins that are conjugated to phosphatidylethanolamine during autophagy. These processes can be inhibited by this vinyl sulfone derivative which reacts with the Apg4B active site cysteine. The HA-epitope allows for the sensitive identification or purification of such deconjugating activities since it is specifically recognized by anti-HA antibodies and/or anti-HA-agarose. Add directly to
in vitro
assay from the stock solution. Depending on conditions and detection method, typical concentrations to fully inhibit Apg deconjugating enzymes
in vitro
are 1-5μM.



Declaration

Manufactured by Boston Biochem



Shipping and Handling



Shipping

DRY ICE



Short Term Storage

-20°C



Long Term Storage

-80°C



Handling Advice

Aliquot to avoid freeze/thaw cycles.



Use/St
ABI
lity

Stable for at least 1 year after receipt when stored at -80°C.



Documents



MSD
S

No



Product Specification Sheet



Datasheet


Download PDF





Golgi-associated ATPase Enhancer of 16 kDa (GATE-16), also known as Apg8p2 and GABARAPL2, is a 117 amino acid (aa) polypeptide and a member of the autophagy-related 8 (Atg8) family of proteins. GATE-16 has 100% aa sequence identity with its mouse and rat orthologs, and is orthologous to the yeast Atg8. Atg8 family members show structural similarity with ubiquitin, but lack aa sequence similarity. GATE-16 is best known for its role in autophagy. GATE-16 covalently attaches to phosphatidylethanolamine (PE) the phagophore (autophagosome precursor) membrane using a ubiquitin-like conjugation system that includes ubiquitin-activating (E1)-, ubiquitin-conjugating (E2)-, and ubiquitin ligase (E3)-like enzymes. Here it is involved in the later stages of autophagosome formation. It may also be involved in cargo recruitment to autophagosomes.