商品编号


AG-40T-0266-R250



品牌


Adipogen



公司


Adipogen



公司分类


Proteins



Size

250 ?l

商品信息


More Information



Product Details



Synonyms

UV Excision Repair Protein RAD23 Homolog A



Product Type

Protein



Properties



Source/Host

E. coli



Sequence

Human HR23A (TUBE1 aa 161-358) (Accession Nr. P54725-1) is conjugated to agarose.



Crossreactivity

Human



Label/Conjugates

Agarose



Formulation

Liquid. In 50mM HEPES, pH 7.5, 250mM NaCl.



Other Product Data


Use:
This affinity resin can be used for the enrichment, isolation and identification of K63-linked (preferentially) or K48-linked poly-ubiquitin chains or ubiquitinated substrates that contain these linkages. Equilibrate resin by washing with 5-10ml desired start buffer. Binding and elution of material is dependent on individual experimental conditions.

Storage:
The agarose can be re-used for at least 2 applications if properly maintained. After use, clean resin with 5ml 50mM Tris pH 9.0, 1M KCl. Remove cleaning solution by washing resin with 5ml storage buffer.



Declaration

Manufactured by Boston Biochem



Shipping and Handling



Shipping

BLUE ICE



Short Term Storage

+4°C



Long Term Storage

+4°C



Handling Advice

Do not freeze.



Use/St
ABI
lity

Stable for at least 3 months after receipt when stored at +4°C.



Documents



MSD
S

No



Product Specification Sheet



Datasheet


Download PDF





Human HR23A/Rad23A has two ubiquitin-associated (UBA) motifs that can each bind ubiquitin via a hydrophobic surface formed by residues located within the α1 and α3 helices of each UBA domain. Tandem-repeated ubiquitin binding entities (TUBEs), which consist of multiple tandem ubiquitin-binding UBA motifs, have been developed for the isolation and identification of ubiquitinated proteins. TUBEs show increased affinity for poly-ubiquitin moieties compared to single UBA motifs. Additionally, TUBEs protect polyubiquitinated proteins from deubiquitylating enzymes, allowing for the detection of polyubiquitinated proteins at relatively low levels of abundance.