商品编号


CHI-HF-20110-C010



品牌


Adipogen



公司


Adipogen



公司分类


Proteins



Size

10 ?g

商品信息


More Information



Product Details



Synonyms

Interleukin-10; Cytokine Synthesis Inhibitory Factor; CSIF



Product Type

Protein



Properties



Source/Host

HEK 293 cells



Sequence

Human IL-10 (aa 19-178) is fused at the C-terminus to a His-tag.



Crossreactivity

Human



BIOLOG
ical Activity

Determined by the dose-dependent co-stimulation (with human IL-4) of MC/9 cells. The ED50 was found to be ≤ 2.0 ng/ml, corresponding to a specific activity of ≥ 5 x 10
5
units/mg.



MW

~18kDa (SDS-PAGE)



Purity

≥95% (SDS-PAGE)



Endotoxin Content

<0.01eu mu;g="" protein="" (lal="" test;="" lonza).="">0.01eu>



Reconstitution

Reconstitute 10?g vial with 100 ?l sterile water to a concentration of 0.1mg/ml.
Reconstitute 50?g vial with 100 ?l sterile water to a concentration of 0.5mg/ml.
Add 1X PBS to the desired protein concentration.



Formulation

Lyophilized from 0.2μm-filtered solution in PBS.



Other Product Data

NCBI reference NP_000563.1: IL-10 (human)



Declaration

Manufactured by Chimerigen.



Shipping and Handling



Shipping

BLUE ICE



Short Term Storage

+4°C



Long Term Storage

-20°C



Handling Advice

Avoid freeze/thaw cycles.
PBS containing at least 0.1% BSA should be used for further dilutions.



Use/St
ABI
lity

Stable for at least 1 year after receipt when stored at -20°C.
Working aliquots are stable for up to 3 months when stored at -20°C.



Documents



MSD
S

No



Product Specification Sheet



Datasheet


Download PDF





Interleukin-10 (IL-10) is a cytokine produced by activated Th2 cells, B cells, keratinocytes and monocytes/macrophages.
In vitro
murine and human IL-10 inhibits cytokine synthesis by Th1 cells, natural killer cells and monocytes/macrophages. Several studies have suggested the potential application of IL-10 as an anti-inflammatory agent in the treatment of septic shock and as an immunosuppressive agent in certain T cell mediated autoimmune diseases.