商品编号


CHI-HR-20018-C010



品牌


Adipogen



公司


Adipogen



公司分类


Proteins



Size

10 ?g

商品信息


More Information



Product Details



Synonyms

Interleukin-18; Interferon-γ-inducing Factor; IGIF; IL-1γ; IL1F4



Product Type

Protein



Properties



Source/Host

E. coli



Sequence

Human IL-18 (aa 37-193) is fused at the N-terminus to a His-tag.



Crossreactivity

Human



MW

~18kDa



Purity

≥97% (SDS-PAGE)



Endotoxin Content

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Reconstitution

Reconstituted in sterile H
2
O not less than 100μg/ml, which can then be further diluted in other aqueous solutions.



Formulation

Lyophilized from 0.2μm-filtered solution in 1X PBS.



Other Product Data

NCBI reference NP_001553.1: IL-18 (human)



Declaration

Manufactured by Chimerigen.



Shipping and Handling



Shipping

BLUE ICE



Short Term Storage

+4°C



Long Term Storage

-20°C



Handling Advice

Avoid freeze/thaw cycles.



Use/St
ABI
lity

Stable for at least 1 year after receipt when stored at -20°C.
Working aliquots are stable for up to 3 months when stored at -20°C.



Documents



MSD
S

No



Product Specification Sheet



Datasheet


Download PDF





Interleukin-18 (IL-18) is a costimulatory factor for production of interferon-γ (IFN-γ) in response to toxic shock and shares functional similarities with IL-12. IL-18 is synthesized as a precursor 24kDa molecule without a signal peptide and must be cleaved to produce an active molecule. IL-1 converting enzyme (ICE; Caspase-1) cleaves pro-IL-18 at aspartic acid in the P1 position, producing the mature, bioactive peptide that is re
ADI
ly released from the cells. It is reported that IL-18 is produced from Kupffer cells, activated macrophages, keratinocytes, intestinal epithelial cells, osteoblasts, adrenal cortex cells and murine diencephalon. IFN-γ is produced by activated T or NK cells and plays critical roles in the defense against microbiral pathogens. IFN-γ activates macrophages and enhances NK activity and B cell maturation, proliferation and Ig secretion. IFN-γ also induces expression of MHC class I and II antigens and inhibits osteoclast activation. IL-18 acts on T helper type-1 (Th1) T cells and in combination with IL-12 strongly induces them to produce IFN-γ. Pleiotropic effects of IL-18 have also been reported, such as enhancement production of IFN-γ and GM-CSF in peripheral blood mononuclear cells, production of Th1 cytokines, IL-2, GM-CSF, IFN-γ in T cells and enhancement of Fas ligand expression by Th1 cells.