商品编号


CHI-HF-22002-C050



品牌


Adipogen



公司


Adipogen



公司分类


Proteins



Size

50 ?g

商品信息


More Information



Product Details



Synonyms

Interleukin-2; Aldesleukin



Product Type

Protein



Properties



Source/Host

NS1 cells



Sequence

The extracellular domain of human IL-2 (aa 21-153) is fused to the N-terminus of the Fc region of a mutant human IgG1.



Crossreactivity

Human



BIOLOG
ical Activity

Shows the
BIOLOG
ical function of the IL-2 moiety and exerts a prolonged circulating half-life caused by the modified Fc domain.



Purity

≥98% (SDS-PAGE)



Endotoxin Content

<0.06eu mu;g="" protein="" (lal="" test;="" lonza).="">0.06eu>



Reconstitution

Reconstitute in 50?l sterile water.
Add 1X PBS to the desired protein concentration.



Formulation

Lyophilized from 0.2μm-filtered solution in PBS.



Other Product Data

Non-lytic: Acts as a long lasting fusion protein which only binds to the receptor. Mutations to the complement (C1q) and FcgR I binding sites of the IgGs Fc fragment render the fusion proteins incapable of antibody directed cytotoxicity (ADCC) and complement directed cytotoxicity (CDC).
NCBI reference NP_000577.2: IL-2 (human)



Declaration

Manufactured by Chimerigen.



Shipping and Handling



Shipping

BLUE ICE



Short Term Storage

+4°C



Long Term Storage

-20°C



Handling Advice

Avoid freeze/thaw cycles.



Use/St
ABI
lity

Stable for at least 1 year after receipt when stored at -20°C.
Working aliquots are stable for up to 3 months when stored at -20°C.



Documents



MSD
S

No



Product Specification Sheet



Datasheet


Download PDF





Interleukin-2 (IL-2) is a 133 amino acid glycoprotein with one intramolecular disulfide bond and variable glycosylation. It is secreted by activated T cells and induces proliferation and maturation of activated T cells, natural killer cells, and lymphokine activated killer cells. IL-2 also stimulates proliferation of antibody-producing B cells, activates neutrophils, and induces mononuclear cells to secrete IFN-γ and TNF-α and -β. Moreover, studies have shown that IL-2 is required for activation-induced apoptosis, an important hemeostatic mechanism in the immune system, which is involved in the maintenance of peripheral tolerance to self-antigens.


Product References

Augmentation of immune responses to HIV-1 and simian immunodeficiency virus DNA vaccines by IL-2/Ig plasmid admi
NIST
ration in rhesus monkeys: D.H. Barouch, et al.; PNAS
97,
4192 (2000)

Control of viremia and prevention of clinical AIDS in Rhesus monkeys by cytokine-augmented DNA vaccination: D.H. Barouch, et al.; Science
290,
486 (2000)

Vaccine-elicited immune responses prevent clinical AIDS in SHIV(89.6P)-infected rhesus monkeys: D.H. Barouch, et al.; Immunol. Lett.
79,
57 (2001)