商品编号


CHI-MF-12002-C010



品牌


Adipogen



公司


Adipogen



公司分类


Proteins



Size

10 ?g

商品信息


More Information



Product Details



Synonyms

Interleukin-2; Aldesleukin



Product Type

Protein



Properties



Source/Host

NS1 cells



Sequence

The extracellular domain of mouse IL-2 (aa 21-169) is fused to the N-terminus of the Fc region of a mutant mouse IgG2a.



Crossreactivity

Mouse



BIOLOG
ical Activity

Specific Activity: 1-1.5 x10
6
Units/mg, measured using CTLL indicator cells.
Shows the
BIOLOG
ical function of the IL-2 moiety and exerts a prolonged circulating half-life caused by the modified Fc domain. Blocks diabetogenic autoimmunity in nonobese diabetic mice.



Purity

≥98% (SDS-PAGE)



Endotoxin Content

<0.06eu mu;g="" protein="" (lal="" test;="" lonza).="">0.06eu>



Reconstitution

Reconstitute at 100μg/ml in sterile PBS.



Formulation

Lyophilized from 0.2μm-filtered solution in PBS.



Other Product Data

Non-lytic: Acts as a long lasting fusion protein which only binds to the receptor. Mutations to the complement (C1q) and FcgR I binding sites of the IgGs Fc fragment render the fusion proteins incapable of antibody directed cytotoxicity (ADCC) and complement directed cytotoxicity (CDC).
NCBI reference NP_032392.1: IL-2 (mouse)



Declaration

Manufactured by Chimerigen.



Shipping and Handling



Shipping

BLUE ICE



Short Term Storage

+4°C



Long Term Storage

-20°C



Handling Advice

Avoid freeze/thaw cycles.



Use/St
ABI
lity

Stable for at least 1 year after receipt when stored at -20°C.
Working aliquots are stable for up to 3 months when stored at -20°C.



Documents



MSD
S

No



Product Specification Sheet



Datasheet


Download PDF





Interleukin-2 (IL-2) is a 133 amino acid glycoprotein with one intramolecular disulfide bond and variable glycosylation. It is secreted by activated T cells and induces proliferation and maturation of activated T cells, natural killer cells, and lymphokine activated killer cells. IL-2 also stimulates proliferation of antibody-producing B cells, activates neutrophils, and induces mononuclear cells to secrete IFN-γ and TNF-α and -β. Moreover, studies have shown that IL-2 is required for activation-induced apoptosis, an important hemeostatic mechanism in the immune system, which is involved in the maintenance of peripheral tolerance to self-antigens.


Product References

Admi
NIST
ration of noncytolytic IL-10/Fc in murine models of lipopolysaccharide-induced septic shock and allogeneic islet transplantation: X.X. Zheng, et al.; J. Immunol.
154,
5590 (1995)

Augmentation and suppression of immune responses to an HIV-1 DNA vaccine by plasmid cytokine/Ig admi
NIST
ration: D.H. Barouch, et al.; J. Immunol.
161,
1875 (1998)

IL-2 receptor-targeted cytolytic IL-2/Fc fusion protein treatment blocks diabetogenic autoimmunity in nonobese diabetic mice: X.X. Zheng, et al.; J. Immunol.
163,
4041 (1999)

Effects of Cyclosporine on Transplant Tolerance: The Role of IL-2: X.X. Zheng, et al.; Am. J. Transplant.
7,
1907 (2007)

Single-cell quantification of IL-2 response by effector and regulatory T cells reveals critical plasticity in immune response: O. Feinerman, et al.; Mol. Sys. Biol.
6,
437 (2010)