商品编号


AG-40T-0275-R500



品牌


Adipogen



公司


Adipogen



公司分类


Proteins



Size

500 ?l

商品信息


More Information



Product Details



Synonyms

ISG15 Ubiquitin-like Modifier; G1P2; IFI15; IP17; UCRP



Product Type

Protein



Properties



Source/Host

E. coli



Sequence

Human ISG15 (Accession Nr. P05161) coupled to agarose.



Crossreactivity

Human



Label/Conjugates

Agarose



Formulation

Liquid. In 50mM HEPES, 250mM NaCl and 0.09% (w/v) Sodium Azide, pH 7.5.



Other Product Data


Use:
Covalently coupled to agarose beads via primary amines allowing for a fully functional C-terminus. Ideal for the enrichment of known ISG15-interacting proteins as well as the discovery of novel ISG15-interacting proteins. We recommend equilibrating the resin by washing with 5-10ml of your desired aqueous buffer.



Declaration

Manufactured by Boston Biochem



Shipping and Handling



Shipping

BLUE ICE



Short Term Storage

+4°C



Long Term Storage

+4°C



Handling Advice

Do not freeze.



Use/St
ABI
lity

Stable for at least 3 months after receipt when stored at +4°C.



Documents



MSD
S

No



Product Specification Sheet



Datasheet


Download PDF





Interferon-stimulated Gene 15 (ISG15), also known as UCRP, is a ubiquitin-like protein that is covalently coupled to target proteins in a process termed ISGylation. It is a 165 amino acid (aa) polypeptide with a predicted molecular weight of 18 kDa. ISG15 exhibits 66% aa sequence identity with its mouse ortholog. Structurally, ISG15 consists of two tandem ubiquitin-like domains that share a similar 3-dimensional structure with ubiquitin and other ubiquitin-like modifiers including NEDD8 and SUMO1. Modification of targets by ISG15 occurs in a stepwise enzymatic process similar to that of ubiquitin. Enzymes regulating ISGylation include the activating (E1) enzyme UBE1L, the conjugating (E2) enzyme UbcH8, and ligases (E3) such as TRIM25 and HERC5. Removal of ISG15 is catalyzed by the deconjugating enzyme UBP43/
USP
18. Functionally, ISG15 has putative roles in the immune response and tum
Origen
esis. This is reflected by intracellular ISG15 targets that include Cyclin D1, tumor suppressor p63, IRF3 and a range of viral proteins. It is induced by type 1 interferons and microbial infection and knockout mice exhibit an increased sensitivity to infection by some viruses. ISG15 can also be secreted by cells of the immune system and may act in a cytokine-like manner. For instance, it is produced by human granulocytes in response to mycobacterium exposure and natural killer cells and T cells respond to extracellular ISG15 with IFN-γ production. Further supporting a role in immune function, ISG15 mutations are associated with MSMD, an inherited disorder characterized by increased susceptibility to mycobacterial infection.