商品编号


AG-40T-0279-C050



品牌


Adipogen



公司


Adipogen



公司分类


Proteins



Size

50 ?g

商品信息


More Information



Product Details



Synonyms

Ubiquitin-like Modifier Activating Enzyme 7; UBE2; Ubiquitin-activating Enzyme 7; D8; UBA1B; UBA7; UBE1L



Product Type

Protein



Properties



Source/Host

Sf9 cells



Sequence

Human ISG15 E1 (Accession Nr. P41226) fused to a N-terminal GST-tag.



Crossreactivity

Human



Formulation

Liquid. In HEPES and NaCl.



Other Product Data


Use:
Member of the ISG15-activating (E1) enzyme family that is required for the first step of the enzymatic cascade that subsequently utilizes a ISG15-conjugating (E2) enzyme and a ISG15 ligase (E3) to conjugate ISG15 to substrate proteins. Reaction conditions will need to be optimized for each specific application. We recommend an initial protein concentration of 50-200nM.



Declaration

Manufactured by Boston Biochem



Shipping and Handling



Shipping

DRY ICE



Short Term Storage

-20°C



Long Term Storage

-80°C



Handling Advice

Aliquot to avoid freeze/thaw cycles.



Use/St
ABI
lity

Stable for at least 1 year after receipt when stored at -80°C.



Documents



MSD
S

No



Product Specification Sheet



Datasheet


Download PDF





Interferon-stimulated Gene 15 (ISG15) Activating Enzyme (E1), also known as ubiquitin-activating enzyme1-like (UBE1L), UBA7, and UBE2, is a 1012 amino acid (aa) member of the ubiquitin-activating enzyme family with a predicted molecular weight of 111 kDa. The mouse and rat ISG15 E1/UBE1L orthologs share 80% aa sequence identity with the human protein. ISG15 E1/UBE1L catalyzes the activation of the C-terminal carboxyl group of the small ubiquitin-like modifier ISG15. Like other E1 enzymes, ISG15 E1/UBE1L contains a conserved ATP-binding domain and an active site cysteine residue, Cys599 in humans. It is expressed in multiple tissues in response to IFN-α or -β, but the covalent attachment of ISG15 to protein substrates (ISGylation) is not required for IFN-α or -β signaling. In addition, ISG15 E1/UBE1L knockout mice appear to have normal embryonic development and fertility. However, it has been suggested that ISG15 E1/UBE1L may play a role in viral immunity as these knockout mice demonstrate increased susceptibility to influenza infection. ISG15 E1/UBE1L has also been shown to modulate erythroid differentiation and mediate retinoid-induced differentiation of acute promyelocytic leukemia cells. ISG15 E1/UBEL1 is downregulated in lung cancer and has been shown to downregulate Cyclin D in lung cancer cells.