商品编号


AG-40T-0107-C050



品牌


Adipogen



公司


Adipogen



公司分类


Proteins



Size

50 ?g


商品信息


More Information



Product Details



Synonyms

Probable Inorganic Polyphosphate/ATP-NAD kinase; Poly(P)/ATP NAD Kinase 1; ppnK1; EC 2.7.1.23



Product Type

Protein



Properties



Source/Host

E. coli



Sequence

NAD kinase (
Mycobacterium tuberculosis
) is fused at the N-terminus to a His-tag.



BIOLOG
ical Activity

~1.2 U/mg protein. (Substrate: NAD (+ ATP))
~3.8 U/mg protein. (Substrate: NAD (+ poly(P))
One unit is defined as the amount of enzyme that synthesizes 1?mol nicotinamide adenine dinucleotide phosphate (NADP) per min.



MW

~33.0kDa



Purity

≥98% (SDS-PAGE)



Endotoxin Content

N/A



Concentration

0.5mg/ml (Lot specific)



Formulation

Liquid. In 10mM TRIS at pH 8.0, containing 1mM MgCl
2
, 0.5mM EDTA, 1mM DTT and 1mM PMSF.



Other Product Data

UniProt link P0A5S6: NAD Kinase (M. tuberculosis)



Shipping and Handling



Shipping

DRY ICE



Short Term Storage

-20°C



Long Term Storage

-80°C



Handling Advice

After opening, prepare aliquots and store at -80°C.
Avoid freeze/thaw cycles.



Use/St
ABI
lity

Stable for at least 6 months after receipt when stored at -80°C.



Documents



MSD
S


Download PDF



Product Specification Sheet



Datasheet


Download PDF





NAD kinase catalyzes the transfer of a phosphate group from ATP to NAD
+
to generate NADP
+
, which in its reduced form acts as an electron donor for biosynthetic reactions. NADP
+
is an essential coenzyme in metabolism and provides reducing power to biosynthetic processes such as fatty acid biosynthesis. NAD kinase from
Mycobacterium tuberculosis
is not ATP-dependent but has a broader substrate specificity than human NAD kinase. It can economically produce NADP
+
by using other nucleoside triphosphates as well as inorganic polyphosphate as a source of phosphorus. Was identified as a high-confidence drug target.
Catalytic activity: ATP + NAD
+
= ADP + NADP
+



Product References

Characterization of Mycobacterium tuberculosis NAD Kinase: Functional Analysis of the Full-Length Enzyme by Site-Directed Mutagenesis: N. Raffaelli, et al.; Biochemistry
43,
7610 (2004)

A Novel Fold Revealed by Mycobacterium tuberculosis NAD Kinase, a Key Allosteric Enzyme in NADP Biosynthesis: S. Garavaglia, et al.; J. Biol. Chem.
279,
40980 (2004)