商品编号


AG-40T-0110-C050



品牌


Adipogen



公司


Adipogen



公司分类


Proteins



Size

50 ?g


商品信息


More Information



Product Details



Synonyms

NadN; NAD Pyrophosphatase/5'-nucleotidase



Product Type

Protein



Properties



Source/Host

E. coli



Sequence

Full-length NAD 5’-nucleotidase (
Haemophilus influenzae
) without the signal peptide corresponding to the first 26 aa.



BIOLOG
ical Activity


With AMP:
856.4 nmol of Ade/min/mg of protein

With NMN:
110,3 nmol of NR/min/mg of protein

With NAD:
5.4 nmol of Ade/min per mg of protein | 5.3 nmol of NR/min/mg of protein



MW

~63.0kDa



Purity

≥98% (SDS-PAGE)



Endotoxin Content

N/A



Concentration

0.5mg/ml (Lot specific)



Formulation

Liquid. In 5mM KH
2
PO
4
, pH 6.8 containing 100mM TRIS-HCl (pH 8.5) and 10mM MgCl
2
.



Other Product Data

UniProt link Q4QNY4: NAD Nucleotidase (H. influenza)



Shipping and Handling



Shipping

DRY ICE



Short Term Storage

-20°C



Long Term Storage

-80°C



Handling Advice

After opening, prepare aliquots and store at -80°C.
Avoid freeze/thaw cycles.



Use/St
ABI
lity

Stable for at least 6 months after receipt when stored at -80°C.



Documents



MSD
S


Download PDF



Product Specification Sheet



Datasheet


Download PDF





NadN (NAD nucleotidase) is a periplasmic enzyme from
Haemophilus influenzae
, a major pathogen of the respiratory tract in humans that has developed the cap
ABI
lity to exploit host NAD(P) for its nicotinamide dinucleotide requirement. NadN plays a central role by degr
ADI
ng NAD into adenosine and NR (nicotinamide riboside), NMN to nicotinamide riboside and AMP to adenosine. It shows a broad substrate specificity, recognizing either mono- or di-nucleotide nicotinamides and different adenosine phosphates with a maximal activity on 5'-adenosine monophosphate. It can be used as an economically alternative to produce NR (nicotinamide riboside).
Catalytic activity: NAD=AMP + NMN=Ade+NR+PPi | AMP=Ade+Pi | NMN=NR+Pi


Product References

The high-resolution crystal structure of periplasmic Haemophilus influenzae NAD nucleotidase reveals a novel enzymatic function of human CD73 related to NAD metabolism: S. Garavaglia, et al.; Biochem. J.
441,
131 (2012)