商品编号


AG-40T-0105-C050



品牌


Adipogen



公司


Adipogen



公司分类


Proteins



Size

50 ?g


商品信息


More Information



Product Details



Synonyms

Nicotinate Phosphoribosyltransferase; FHA-HIT-interacting Protein; FHIP; EC 2.4.2.11



Product Type

Protein



Properties



Source/Host

E. coli



Sequence

Human NAPRTase is fused at the N-terminus to a His-tag.



Crossreactivity

Human



BIOLOG
ical Activity

~0.27 U/mg protein. One unit is defined as the amount of enzyme that synthesizes 1?mol nicotinic acid mononucleotide (NaMN) per min.



MW

~58.0kDa



Purity

≥98% (SDS-PAGE)



Endotoxin Content

N/A



Concentration

0.5mg/ml (Lot specific)



Formulation

Liquid. In 50mM HEPES/KOH pH 7.5 containing 300mM potassium chloride and 10mM DTT.



Other Product Data

UniProt link Q6XQN6: NAPRTase (human)



Shipping and Handling



Shipping

DRY ICE



Short Term Storage

-20°C



Long Term Storage

-80°C



Handling Advice

After opening, prepare aliquots and store at -80°C.
Avoid freeze/thaw cycles.



Use/St
ABI
lity

Stable for at least 6 months after receipt when stored at -80°C.



Documents



MSD
S


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Product Specification Sheet



Datasheet


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Human nicotinate phosphoribosyltransferase (NAPRTase) is localized in the cytoplasm and is involved in
BIOLOG
ical processes such as NAD biosynthetic and metabolic processes, nicotinamide metabolic process, nicotinate nucleotide salvage, response to oxidative stress and water-soluble vitamin metabolic process. It functions by catalyzing the conversion of nicotinic acid (NA) to NA mononucleotide (NaMN) and is essential for NA to increase cellular NAD levels and prevent oxidative stress of the cells. It is a crucial factor in the NAD
+
biosynthesis pathway.
Catalytic activity: Β-nicotinate D-ribonucleotide + diphosphate = nicotinate + 5-phospho-α-D-ribose 1-diphosphate.


Product References

Characterization of human nicotinate phosphoribosyltransferase: Kinetic studies, structure prediction and functional analysis by site-directed mutagenesis: L. Galassi, et al.; Biochimie
94,
300 (2012)