商品编号


AG-40T-0295-R500



品牌


Adipogen



公司


Adipogen



公司分类


Proteins



Size

500 ?l

商品信息


More Information



Product Details



Synonyms

Neural Precursor Cell Expressed Developmentally Down-Regulated Protein 8; Neddylin; Rub1



Product Type

Protein



Properties



Source/Host

E. coli



Sequence

Human NEDD8 (Accession Nr. Q15843) coupled to agarose.



Crossreactivity

Human



Label/Conjugates

Agarose



Concentration

5mg/ml resin (0.55mM).



Formulation

Liquid. In 50mM Hepes pH 7.5, 150mM NaCl, 1mM azide.



Other Product Data


Use:
Covalently coupled to agarose beads via primary amines allowing for a fully functional C-terminus. Ideal for the enrichment of known NEDD8-interacting proteins as well as the discovery of novel NEDD8-interacting proteins. We recommend equilibrating the resin by washing with 5-10ml of your desired aqueous buffer.



Declaration

Manufactured by Boston Biochem



Shipping and Handling



Shipping

BLUE ICE



Short Term Storage

+4°C



Long Term Storage

+4°C



Handling Advice

Do not freeze.



Use/St
ABI
lity

Stable for at least 3 months after receipt when stored at +4°C.



Documents



MSD
S

No



Product Specification Sheet



Datasheet


Download PDF





Neural Precursor Cell Expressed Developmentally Downregulated Gene 8 (NEDD8), also known as Rub1, is a 6-8 kDa member of the ubiquitin family of proteins. Human pro-NEDD8 is 81 amino acids (aa) in length. Pro-NEDD8 contains one ubiquitin-like domain (aa 1-65) and a 4-5 aa C-terminal propeptide. Cleavage of the propeptide exposes a C-terminal glycine residue that is used to generate a glycine-lysine intermolecular bond. Mature human NEDD8 shows 100% aa identity to NEDD8 from mouse, rat and canine. Human NEDD8 is activated by a distinct NEDD8-activating (E1) enzyme, a heterodimeric complex composed of APPBP1 and UBA3 subunits. Activated NEDD8 is subsequently transferred to the UBE2M/Ubc12 or UBE2F NEDD8-conjugating (E2) enzymes. Through a process termed neddylation, the ROC1/Rbx1 RING Finger E3 ligase transfers NEDD8 to specific substrates. For example, transfer of NEDD8 to the cullin scaffold activates members of the Cullin-RING Ligase (CRL) family of ubiquitin ligases (E3). CRL-induced ubiquitination is known to regulate proteins that are important for cell cycle progression and cell survival. Physiologically, NEDD8 plays a critical regulatory role in cell proliferation and dysregulation of the NEDD8 pathway has been associated with several cancer pathologies.