商品编号


AG-40T-0303-C100



品牌


Adipogen



公司


Adipogen



公司分类


Proteins



Size

100 ?g

商品信息


More Information



Product Details



Synonyms

EHEC Type III Effector NleL



Product Type

Protein



Properties



Source/Host

E. coli



Sequence

E. coli O157:H7 NleL (catalytic domain 170-782) (Accession Nr. Q8X5G6).



Crossreactivity

E. coli



Formulation

Liquid. In HEPES, NaCl, Glycerol and TCEP.



Other Product Data


Use:
Bacterial HECT class ubiquitin Ligases (E3) that catalyzes the formation of mixed K6 and K48-linked unanchored polyubiquitin chains. Reaction conditions will need to be optimized for each specific application. We recommend an initial protein concentration of 0.1-0.5?M.



Declaration

Manufactured by Boston Biochem



Shipping and Handling



Shipping

DRY ICE



Short Term Storage

-20°C



Long Term Storage

-80°C



Handling Advice

Aliquot to avoid freeze/thaw cycles.



Use/St
ABI
lity

Stable for at least 1 year after receipt when stored at -80°C.



Documents



MSD
S

No



Product Specification Sheet



Datasheet


Download PDF





NleL (Non-Lee-encoded effector Ligase, also known as espX7) is an effector protein translocated into host cells via the type III secretion system of E. coli O157:H7, a widespread pathogen causing severe foodborne diseases. Although the
BIOLOG
ical relevance of NleL is presently unknown, recent studies clearly demonstrate that this protein is functionally related to the HECT (Homologous to E6AP Carboxy Terminus) class E3 ubiquitin ligases of higher eukaryotes. NleL shows no primary sequence homology to any eukaryotic proteins, but biochemical and structural studies revealed both N-terminal and C-terminal lobes connected by a flex
IBL
e linker, and a catalytic cysteine that accepts ubiquitin from E2 carrier proteins, both hallmarks of HECT domain ligases. NleL catalyzes the formation of mixed K6 and K48-linked unanchored polyubiquitin chains.