商品编号


AG-40A-0074-C010



品牌


Adipogen



公司


Adipogen



公司分类


Proteins



Size

10 ?g


商品信息


More Information



Product Details



Synonyms

DNA-binding Protein NEFA; Prepronesfatin; Nucb2; Nesfatin



Product Type

Protein



Properties



Source/Host

E. coli



Sequence

Mouse nucleobindin-2 (aa 25-420) is fused at the C-terminus to a His-tag.



Crossreactivity

Mouse



MW

~55kDa (SDS-PAGE)



Purity

≥90% (SDS-PAGE)



Endotoxin Content

<1eu mu;g="" purified="" protein="" (lal="" test;="" lonza).="">1eu>



Formulation

Liquid. 0.2μm-filtered solution in PBS, pH 7.2.



Other Product Data

UniProt link P81117: Nucleobindin-2 (mouse) [Precursor]



Shipping and Handling



Shipping

BLUE ICE



Short Term Storage

+4°C



Long Term Storage

-20°C



Handling Advice

After opening, prepare aliquots and store at -20°C.
Avoid freeze/thaw cycles.



Use/St
ABI
lity

Stable for at least 6 months after receipt when stored at -20°C.
Working aliquots are stable for up to 3 months when stored at -20°C.



Documents



MSD
S


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Product Specification Sheet



Datasheet


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Nucleobindin-2 (NUCB2; Prepronesfatin) was first isolated in
ADI
pocytes, but is also expressed by gastric mucosa and pancreatic β cells. This pattern of nucleobindin-2 expression, as well as the presence of nucleobindin-2 within the plasma of rodents and humans, strongly suggests that this compound may act as a circulating regulatory factor. NUCB2 can be cleaved into three polypeptides: nesfatin-1, nesfatin-2, and nesfatin-3. Anorexigenic effects have been described for nesfatin-1 that plays an important role in hypothalamic pathways regulating food intake and energy homeostasis and acting in a leptin-independent manner. Nesfatin-1 may also exert hypertensive roles and modulate blood pressure through directly acting on peripheral arterial resistance. Nucleobindin-2 is therefore reported to reduce food intake after admi
NIST
ration, mostly refering to the cleaved peptide nesfatin-1. Excess nucleobindin-2/nesfatin-1 in the brain leads to a loss of appetite, lack of the protein in the brain leads to an increase of appetite.