商品编号


AG-40T-0318-C100



品牌


Adipogen



公司


Adipogen



公司分类


Proteins



Size

100 ?g

商品信息


More Information



Product Details



Synonyms

Proteasome Activator Complex Subunit 3; 11S Regulator Complex Subunit γ; PSME3; REG γ



Product Type

Protein



Properties



Source/Host

E. coli



Sequence

Human PA28 Activator (γ-subunit) (Accession Nr. P61289) fused to a N-terminal His-tag.



Crossreactivity

Human



Purity

≥95% (SDS-PAGE)



Formulation

Liquid. In 50mM HEPES, pH8.0, 0.5M NaCl, 0.5mM EDTA and 1mM TCEP.



Other Product Data


Use:
Ideal for the activation of latent 20S Proteasome complexes. Reaction conditions will need to be optimized for each specific application.



Declaration

Manufactured by Boston Biochem



Shipping and Handling



Shipping

DRY ICE



Short Term Storage

-20°C



Long Term Storage

-80°C



Handling Advice

Aliquot to avoid freeze/thaw cycles.



Use/St
ABI
lity

Stable for at least 1 year after receipt when stored at -80°C.



Documents



MSD
S

No



Product Specification Sheet



Datasheet


Download PDF





The Proteasome Activator 28 (PA28) Regulatory Complex, also known as the 11S regulatory (REG) complex, is a ring-shaped, multimeric, ATP-independent regulatory complex that can bind to one or both ends of the 20S Proteasome or 20S Immunoproteasome. The PA28 regulatory complex stimulates the proteasome to hydrolyze small peptides, but not ubiquitinated proteins, for the production of peptides for MHC class I antigen presentation. Three PA28 regulatory complex subunits, α, β, and γ, have been identified and can oligomerize to form the PA28 regulatory complex. While the expression of the PA28α and β subunits are induced by IFN-γ, the PA28γ subunit is not significantly affected. The human PA28 activator γ subunit, also known as 11S REG γ and Ki antigen, is a 254 amino acid (aa) member of the PA28 family of regulatory proteins with a predicted molecular weight of 29 kDa. The mouse and rat orthologs share 100% aa sequence identity with human PA28 γ. Two isoforms of PA28 γ have been identified. Six PA28 γ subunits combine to form a homo-hexameric ring-shaped PA28 regulatory complex. Unlike PA28 regulatory complexes composed of PA28 α subunits or PA28 α/β subunits, regulatory complexes composed of PA28 γ have narrow substrate specificity and have been shown to cleave only a few
BIOLOG
ically relevant protein substrates including SRC-3, p21/CIP1/CDKN1A, and MafA. PA28 γ is involved in cell cycle progression and in T cell-restricted MHC class I presentation of select antigens.