商品编号


AG-40T-0348-R250



品牌


Adipogen



公司


Adipogen



公司分类


Proteins



Size

250 ?l

商品信息


More Information



Product Details



Synonyms

Receptor Associated Protein 80; RXRIP110; UIMC1; X2HRIP110; BRCA1-A Complex Subunit RAP80; Retinoid X Receptor-interacting Protein 110; Ubiquitin Interaction Motif-containing Protein 1



Product Type

Protein



Properties



Source/Host

E. coli



Sequence

Human RAP80 (UIM domains aa 79-124) (Accession Nr. NP_057374) is conjugated to agarose.



Crossreactivity

Human



Label/Conjugates

Agarose



Formulation

Liquid. In 50mM HEPES, pH 7.5, 250mM NaCl, 1mM sodium azide.



Other Product Data


Use:
This affinity resin can be used for the enrichment, isolation and identification of ubiquitinated proteins that are non-K48-linked. Equilibrate resin by washing with 5-10ml desired start buffer. Binding and elution of material is dependent on individual experimental conditions.

Storage:
The agarose can be re-used for at least 2 applications if properly maintained. After use, clean resin with 5ml 50mM Tris pH 9.0, 1M KCl. Remove cleaning solution by washing resin with 5ml storage buffer.



Declaration

Manufactured by Boston Biochem



Shipping and Handling



Shipping

BLUE ICE



Short Term Storage

+4°C



Long Term Storage

+4°C



Handling Advice

Do not freeze.



Use/St
ABI
lity

Stable for at least 3 months after receipt when stored at +4°C.



Documents



MSD
S

No



Product Specification Sheet



Datasheet


Download PDF





Rap80 (Receptor Associated Protein 80) interacts with BRCA1, a ubiquitin E3-ligase which functions in conjugation with the BARD1 deubiquitinating enzyme. BRCA1 is recruited to DNA damage sites by poly-ubiquitin chains through Rap80 which contains 2 tandem ubiquitin-interacting motifs (UIMs). Rap80 constitutes a protein complex with ABRA1 which interacts with the BRCT domain of BRCA1. Upon DNA damage the Rap80-ABRA1 complex targets the BRCA1-BARD1 complex to K6- and K63-linked poly-Ub chains at these foci. The UIM domains of Rap80 have been shown to have preferential binding to K-6 and K63-linked Ub chains and binds to K48-chain with a much lower efficiency. It is not known if Rap80 UIMs interact with Ub chains linked via K11, K27 or K33. Rap80 has a low affinity for mono-, di- and tri-Ub but binds efficiently to tetra (or greater) ubiquitin chains.