商品编号


AG-40T-0351-C100



品牌


Adipogen



公司


Adipogen



公司分类


Proteins



Size

100 ?g

商品信息


More Information



Product Details



Synonyms

E3 Ubiquitin-protein Ligase RNF31; HOIL-1-Interacting Protein; ZIBRA; RING Finger Protein 31



Product Type

Protein



Properties



Source/Host

E. coli



Sequence

Human RNF31 (catalytic domain aa 699-1072) (Accession Nr. Q96EP0) fused to a N-terminal His- and MBP-tag.



Crossreactivity

Human



Formulation

Liquid. In HEPES, NaCl, Glycerol and TCEP.



Other Product Data


Use:
RING domain ubiquitin ligase (E3) and component of the LUBAC complex that functions downstream of a ubiquitin-activating (E1) enzyme and a ubiquitin-conjugating (E2) enzyme to conjugate ubiquitin to substrate proteins. Useful for generating unanchored linear polyubiquitin chains without the requirement for HOIL-1 or Sharpin. Reaction conditions will need to be optimized for each specific application. We recommend an initial protein concentration of 0.1-0.5?M.



Declaration

Manufactured by Boston Biochem



Shipping and Handling



Shipping

DRY ICE



Short Term Storage

-20°C



Long Term Storage

-80°C



Handling Advice

Aliquot to avoid freeze/thaw cycles.



Use/St
ABI
lity

Stable for at least 1 year after receipt when stored at -80°C.



Documents



MSD
S

No



Product Specification Sheet



Datasheet


Download PDF





RNF31 (HOIL-1 Interacting Protein, also known as HOIP) is a RING domain ubiquitin E3 ligase and component of the LUBAC complex. LUBAC, a large (approximately 600 kDa) heteromultimeric assembly consisting of at least HOIP, HOIL-1, and Sharpin, conjugates linear polyubiquitin chains to various substrates and plays a key role in NF-κB activation and signaling events mediated through TNFα and IFN. Both the catalytic activity and specificity for linear ubiquitin chain formation of LUBAC are contained within the C-terminal RBR (RING-IBR-RING) and LDD (linear ubiquitin chain determining domain) domains of HOIP. HOIL-1 and Sharpin are essential for full-length HOIP activity, but not for HOIP truncation mutants consisting of the RBR and LDD domains.