商品编号


AG-40T-0356-C050



品牌


Adipogen



公司


Adipogen



公司分类


Proteins



Size

50 ?g

商品信息


More Information



Product Details



Synonyms

Sentrin-specific Protease 2; Sentrin/SUMO-specific Protease SENP2; Axam2; SMT3-specific Isopeptidase 2



Product Type

Protein



Properties



Source/Host

E. coli



Sequence

Human SENP2 (catalytic domain aa 368-593) (Accession Nr. Q9HC62) fused to a N-terminal His-tag.



Crossreactivity

Human



MW

~29kDa



Purity

≥95% (SDS-PAGE)



Formulation

Liquid. In 50mM HEPES pH8, 100mM NaCl, 10% glycerol, 1mM TCEP.



Other Product Data


Use:
SUMO-specific deconjugating enzyme. Reaction conditions will need to be optimized for each specific application. We recommend an initial protein concentration of 50-500nM. 15 minute pre-incubation with 10mM DTT is recommended to achieve maximum activity.



Declaration

Manufactured by Boston Biochem



Shipping and Handling



Shipping

DRY ICE



Short Term Storage

-20°C



Long Term Storage

-80°C



Handling Advice

Aliquot to avoid freeze/thaw cycles.



Use/St
ABI
lity

Stable for at least 1 year after receipt when stored at -80°C.



Documents



MSD
S

No



Product Specification Sheet



Datasheet


Download PDF





SUMO1/Sentrin/SMT3 Specific Peptidase 2 (SENP2) is a member of the SENP family of proteases. SENPs are a group of cysteine-type peptidases that catalyze two essential functions in the SUMO pathways: processing of full-length small ubiquitin-related modifiers (SUMOs) to their mature forms and deconjugation of SUMOs from SUMOylated proteins. SENP2 is 589 amino acids (aa) in length with a predicted molecular weight of 67.9 kDa. Human SENP2 shares 88% and 87% aa sequence identity with the mouse and rat orthologs, respectively. Mammalian SENPs share a conserved C-terminal catalytic domain while the N-terminal domains have no significant similarity. The N-terminal domain of SENP2 contains a nuclear localization signal and a CRM1-dependent nuclear export signal allowing SENP2 to shuttle between the nucleus and cytoplasm. In the nucleus, SENP2 associates with Nup153, a nucleoporin that is located on the nucleoplasmic side of the nuclear pore complex. It is thought that SENP2 functions are regulated by its association with the nuclear pore complex and nucleocytoplasmic shuttling. SENP2 has been shown to deSUMOylate multiple proteins including C/EBPβ, RORα/NR1F1, IRF3, MEF2A and NEMO. Consequently, SENP2 is thought to be critical for the regulation of various cellular processes such as
ADI
pogen
esis, immune responses and cancer cell growth. Additionally, SENP2 deSUMOylates MDM2, allowing MDM2 to bind and ubiquitinate p53. SENP2-mediated regulation of MDM2 appears to be critical for the development of the trophoblast during embryogenesis and maintenance of the genome during stress responses.