商品编号


AG-40B-0168-C010



品牌


Adipogen



公司


Adipogen



公司分类


Proteins



Size

10 ?g


商品信息


More Information



Product Details



Synonyms

Slit Homolog 2 Protein (C fragment)



Product Type

Protein



Properties



Source/Host

HEK 293 cells



Sequence

Human Slit2 (aa 1122-1529) is fused at the C-terminus to a FLAG
?
-tag.



Crossreactivity

Human



BIOLOG
ical Activity

Activates an UCP1-luciferase construct in mouse fat primary cells at a concentration of 20nM.



MW

~55kDa



Purity

≥95% (SDS-PAGE)



Endotoxin Content

<0.01eu mu;g="" purified="" protein="" (lal="" test;="" lonza).="">0.01eu>



Concentration

0.1mg/ml after reconstitution.



Reconstitution

Reconstitute with 100μl sterile water.



Formulation

Lyophilized. Contains PBS.



Other Product Data

Uni-Prot link 0904813: Slit2 (C fragment)



Shipping and Handling



Shipping

BLUE ICE



Short Term Storage

+4°C



Long Term Storage

-20°C



Handling Advice

After reconstitution, prepare aliquots and store at -20°C.
Avoid freeze/thaw cycles.
PBS containing at least 0.1% BSA should be used for further dilutions.



Use/St
ABI
lity

Stable for at least 6 months after receipt when stored at -20°C.



Documents



MSD
S


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Product Specification Sheet



Datasheet


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The Slits are secreted proteins that bind to Robo receptors and play a role in axon guidance and neuronal migration. The Slit family in mice and humans comprises three members: Slit1, Slit2 and Slit3. These are all extracellular matrix proteins of 180-200kDa.
In vivo
, Slit2 is cleaved into 140kDa N-terminal (Slit2-N) and 55-60 kDa C-terminal (Slit2-C) fragments. Only Slit2-N and Slit2 full-length bind the Robo proteins. Recently, it has been shown that Slit2 is highly expressed in
ADI
pose tissues under the control of PRDM16 and cold exposure. Slit2 is posttranslationally cleaved by
ADI
pocytes and the resulting Slit2-C fragment improves glucose homeostasis in mice by activating a
Thermo
genic PKA/cAMP pathway not mediated by β-adrenergic receptors in
ADI
pocytes.