商品编号


AG-40T-0004-C020



品牌


Adipogen



公司


Adipogen



公司分类


Proteins



Size

20 ?g


商品信息


More Information



Product Details



Synonyms

TRAIL; Apo-2L; TNFSF10; CD253



Product Type

Protein



Properties



Source/Host

E. coli



Sequence

The extracellular domain of mouse TRAIL (aa 99-291) is fused at the N-terminus to a His-tag and a linker peptide. The active multimeric conformation is st
ABI
lized by an inserted mutation allowing an additional CC-bridge.



Crossreactivity

Human
Mouse



Specificity

Binds to mouse and less potently to?human TRAIL receptors.



BIOLOG
ical Activity

Induces apoptosis at concentrations of >10ng/ml as tested on human tumor cells. This effect can be efficiently blocked by recombinant mouse TRAIL receptors. This protein does not require a cross-linker (enhancer) for its potent
BIOLOG
ical activity.



MW

~24kDa



Purity

≥95% (SDS-PAGE)



Purity Detail

Affinity purified. Sterile filtered.



Endotoxin Content

<0.01eu mu;g="" purified="" protein="" (lal="" test;="" lonza).="">0.01eu>



Concentration

0.5mg/ml



Formulation

Liquid. In 20mM HEPES, pH 7.4, 300mM NaCl, 0.01% Tween 20, 1% sucrose, 1mM DTT.



Other Product Data

UniProt link P50592: TRAIL (mouse)



Shipping and Handling



Shipping

DRY ICE



Short Term Storage

-20°C



Long Term Storage

-80°C



Handling Advice

Avoid freeze/thaw cycles.



Use/St
ABI
lity

Once thawed it is recommended to prepare appropriate aliquots and to store them at -80°C.
Stable for at least 1 year after receipt when stored at -80°C.



Documents



MSD
S


Download PDF



Product Specification Sheet



Datasheet


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TNF-related apoptosis-inducing ligand (TRAIL; Apo2L;CD253; TNFSF10) is a type II transmembrane protein of about 34kDa. Like most members of the tumor necrosis factor (TNF) superfamily of cytokines TRAIL can be cleaved at the cell surface by metalloproteases to form a soluble molecule. Active TRAIL forms trimers and specifically binds to five distinct receptors: TRAIL-R1 (DR4; Apo2;CD261; TNFRSF10A), TRAIL-R2 (DR5; KILLER; TRICK2A;TRICK2B; CD262; TNFRSF10B), TRAIL-R3 (DcR1;LIT; TRID; CD263; TNFRSF10C), TRAIL-R4 (DcR2; TRUNDD; CD264; TNFRSF10D), and osteoprotegerin (OPG; OCIF; TNFRSF11B). Trimerized TRAIL triggers apoptosis upon ligation of cell surface TRAIL-R1 and/or TRAIL-R2 by inducing the formation of the so-called multiprotein death-inducing signaling complex (DISC).