商品编号


AG-40T-0002-3020



品牌


Adipogen



公司


Adipogen



公司分类


Proteins



Size

3 x 20 ?g


商品信息


More Information



Product Details



Synonyms

TRAIL; Apo-2L; TNFSF10; CD253



Product Type

Protein



Properties



Source/Host

E. coli



Sequence

The extracellular domain of human TRAIL (aa 95-281) is fused at the N-terminus to a His-tag and a linker peptide. The active multimeric conformation is st
ABI
lized by an inserted mutation allowing an additional CC-bridge.



Crossreactivity

Human



Specificity

Binds to human TRAIL receptors.



BIOLOG
ical Activity

Induces apoptosis at concentrations of >1ng/ml.
Note:
Does not require a cross-linking enhancer for its potent
BIOLOG
ical activity. Shows superior activity on human?cell lines that require extensive cross-linking of TRAIL-Rs for killing (e.g. Jurkat).



Purity

≥98% (SDS-PAGE)



Purity Detail

Affinity purified. Sterile filtered.



Endotoxin Content

<0.01eu mu;g="" purified="" protein="" (lal="" test;="" lonza).="">0.01eu>



Concentration

0.5mg/ml



Formulation

Liquid. In 20mM HEPES, pH 7.4, 150mM NaCl, 0.005% Tween 20, 1% sucrose, 1mM DTT.



Other Product Data

In order to retain full activity do not dilute higher than to a concentration of 0.1mg/ml, prepare 20?l aliquots and store at -80°C. Working dilution: 2-100ng/ml.
UniProt link P50591:


Shipping and Handling


Shipping

DRY ICE



Short Term Storage

-20°C



Long Term Storage

-80°C



Handling Advice

Avoid freeze/thaw cycles.



Use/St
ABI
lity

Once thawed it is recommended to prepare appropriate aliquots and to store them at -80°C.
Stable for at least 1 year after receipt when stored at -80°C.



Documents



MSD
S


Download PDF



Product Specification Sheet



Datasheet


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TNF-related apoptosis-inducing ligand (TRAIL; Apo2L;CD253; TNFSF10) is a type II transmembrane protein of about 34kDa. Like most members of the tumor necrosis factor (TNF) superfamily of cytokines TRAIL can be cleaved at the cell surface by metalloproteases to form a soluble molecule. Active TRAIL forms trimers and specifically binds to five distinct receptors: TRAIL-R1 (DR4; Apo2;CD261; TNFRSF10A), TRAIL-R2 (DR5; KILLER; TRICK2A;TRICK2B; CD262; TNFRSF10B), TRAIL-R3 (DcR1;LIT; TRID; CD263; TNFRSF10C), TRAIL-R4 (DcR2; TRUNDD; CD264; TNFRSF10D), and osteoprotegerin (OPG; OCIF; TNFRSF11B). Trimerized TRAIL triggers apoptosis upon ligation of cell surface TRAIL-R1 and/or TRAIL-R2 by inducing the formation of the so-called multiprotein death-inducing signaling complex (DISC).